Control of mRNA stability by a riboswitch

(a) Transcription and translation lead to production of GlcN6P in B. subtilis when GlcN6P concentration is low. (b) When GlcN6P is in high concentration, it binds to riboswitch sequences and generates mRNA cleavage(a) Transcription and translation lead to production of GlcN6P in B. subtilis when GlcN6P concentration is low. (b) When GlcN6P is in high concentration, it binds to riboswitch sequences and generates mRNA cleavage

(a) Low GIcN6p concentration: Riboswitch not active (b) High GIcN6p concentration: Riboswitch active Riboswitch Shine— Dalgarno sequence /Start AUG codon sequence glmS mRNA 5’ 3' Translation 1 Cleavage portion cleavage l glutaminezfructose-6- phosphate amidotransferase Precursor ‘ Low GIcN6P concentration permits transcription of High GlcN6P concentration leads to GlcN6P binding g/mS, translation of its mRNA, and production of to the riboswitch sequences and cleavage of the GlCN6P. mRNA.