Enhancer trapping to reveal expression patterns of endogenous genes

Strategy for generation of enhancer trap linesStrategy for generation of enhancer trap lines

Endogenous regulatory element Enhancer trap DNA TATA box is used as a minimal promoter to recruit basal transcriptional machinery. EDI—Z Endogenous gene X 1 1 RNA — — «o W B-gal expression in pattern driven by endogenous regulatory element Reporter gene (B-gal) marker regulatory sequences. Selectable Randomly insert enhancer trap into genome via transposon or T-DNA vector. Endogenous gene X If enhancer trap disrupts coding region of gene, a loss-of-function allele is created. However, insertion of vector may occur 5’ or 3’ to a gene and still "trap" enhancers without causing a loss-of-function mutation. If enhancer trap DNA is inte- grated near endogenous regu- latory elements, the reporter gene will be expressed in a pattern driven by adjacent