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Why is it necessary to digest a plasmid DNA and a genomic DNA with the same restriction enzyme?

mirchem

wrote...

Posts: 8

Rep:

11 years ago

Why is it necessary to digest a plasmid DNA and a genomic DNA with the same restriction enzyme?

why is it necessary?

Read 606 times

1 Reply

Related Topics

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Solved Why must you use the same restriction enzyme on the cell dna and on the plasmid?
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Replies

daveys

wrote...

#1

11 years ago

I'm guessing you want to transform the genomic DNA with the plasmid DNA? If so, it's because you want the cuts to be mae in the same place, so both sequences are compatible.

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