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Why is it necessary to digest a plasmid DNA and a genomic DNA with the same restriction enzyme?

mirchem

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Posts: 8

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11 years ago

Why is it necessary to digest a plasmid DNA and a genomic DNA with the same restriction enzyme?

why is it necessary?

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daveys

wrote...

#1

11 years ago

I'm guessing you want to transform the genomic DNA with the plasmid DNA? If so, it's because you want the cuts to be mae in the same place, so both sequences are compatible.

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