Yeast Gene

selfstudy

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11 years ago

You have found a yeast gene that appears to encode a DNA polymerase, and you hypothesize that a related gene exists in humans. Describe, in detail, the experiments you would use to test this hypothesis. Be as thorough as possible.

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colleen

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11 years ago

You can test the ability for the DNA polymerase to be able to synthesize DNA under conditions where you replicate a human cell. Then run a gel. If the bands show up it means the DNA polymerase can work with humans, but if it doesn't show up it is likely that the same polymerase does not work in humans or does not exist there. You can also sequence the polymerase and compare it to known human polymerases and see if any match.

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