× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
3
g
1
c
1
Y
1
1
1
Y
1
Z
1
1
c
1
b
1
a
1
Home Q & A Board Biology-Related Homework Help Medicine Immunology
New Topic  
malagonvarney malagonvarney
wrote...
Go to Answer
Posts: 519
Rep: 0 0
5 years ago
Describe how data are collected and displayed in flow cytometry.
 
  What will be an ideal response
Read 58 times
1 Reply
Replies
Answer verified by a subject expert
hetomlinson06hetomlinson06
wrote...
#1
Posts: 389
Rep: 4 0
5 years ago
Sign in or Sign up in seconds to unlock everything for free
In flow cytometry, multiple measurements are taken of each cell (side scatter, forward scatter, fluorescence of
antibody tags). Data from each cell are placed in categories (channels) based on the intensity of the signal. The data
can be manipulated to view one parameter at a time or multiple parameters at one time. If one measurement is
viewed, the typical display is a frequency distribution or histogram showing the number of cells possessing each
category of signal intensity. If more than one measurement is to be viewed simultaneously, the display can be a two
parameter dot plot or scatter plot. Typically, the operator first selects a particular cell population to analyze. Using
forward/side scatter characteristics, the cell population (monocytes, granulocytes, lymphocytes) is selected in a
process called gating, and the histogram/plot display is restricted only to cells in the gate.
This verified answer contains over 140 words.
1

Related Topics

malagonvarney Author
wrote...

5 years ago
this is exactly what I needed
smi3
wrote...

Yesterday
Thanks
Liarec
wrote...

2 hours ago
You make an excellent tutor!
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  979 People Browsing
 260 Signed Up Today
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 214
  
 515
  
 211
Your Opinion
What's your favorite funny biology word?
Votes: 89
Previous poll results: What percentage of nature vs. nurture dictates human intelligence?