Killing of bacteria by UV light:
1. How does UV light kill bacterial cells?
2. Which of the two microbes you used was most sensitive to UV light?
3. Which was most resistant to UV light?
4. Why are some bacteria more resistant to UV light than others?
5. What was the least amount of UV irradiation required to kill all Staphylococcus aureus
6. What was the least amount of UV irradiation required to kill Bacillus spores?
Antiseptics and Disinfectants:
7. Which antiseptic/disinfectant was most effective against Gram positive organisms?
8. Which antiseptic/disinfectant was most effective against Gram negative organisms?
9. What might explain the differences between the effectiveness on Gram positive versus Gram negative organisms?
10. Which organism was most sensitive to antiseptics/disinfectants?
11. Which organism was lease sensitive to antiseptics/disinfectants?
12. Do you think this information might make a difference in a health care setting? WHY?
Antibiotic Sensitivity:
13. Which antibiotic was most effective against Gram positive organisms? WHY might this be?
14. Which antibiotic was most effective against Gram negative organisms? WHY might this be?
15. Are there any “Broad Spectrum” antibiotics present in the list? How do you know they are Broad Spectrum?
16. Why is Mueller-Hinton used as the media rather than TSA?
17. Which of the antibiotics used do you think would be most effective against yeast? Why?
18. How do bacteria become resistant to antibiotics?
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