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PCR REACTION

JFKgravy

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9 years ago

PCR REACTION

I accidentally used 10 ul of master mix/DNA solution instead of 20 ul. The PCR reaction is set to calibrate temperatures for 20 ul of solution. Will my DNA samples be denatured to a point where they can't replicate?

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padre

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9 years ago

Run it, and see what happens. From experience, things like these usually end up messing the end result Frowning Face

Master Mix contains the right amount of Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers.

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