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CooperS CooperS
wrote...
Posts: 63
Rep: 3 0
10 years ago
Hi,

Is V_max affected by conditions like temperature and pH? To me it makes sense they would be, because it should be able to work faster at certain conditions.

Also, since V_max = k[Enzyme] and k is different for all T, V_max should increase with T, right?

A source would be appreciated.

Thanks!
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~Cooper

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wrote...
Educator
10 years ago
Is V_max affected by conditions like temperature and pH? To me it makes sense they would be, because it should be able to work faster at certain conditions.

The effect of pH on the Vmax of an enzyme catalysed reaction may be explained using the, generally true, assumption that only one charged form of the enzyme is optimally catalytic and therefore the maximum concentration of the enzyme-substrate intermediate cannot be greater than the concentration of this species.

Temperature speeds up Vmax to a point, and causes no change to Km.
Source  http://prof.dr.semih.otles.tripod.com/discovery/effectofph.htm
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