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vcunningham22 vcunningham22
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9 years ago
Heres the question typed out. I've attached the screenshot of the question with the structure of the compound. Could you also let me know how you came to the answer? Thank you so much!

An oligomeric protein has a molecular weight of 475000. A 780 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger\'s reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 2.5 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the α-amino groups of other amino acids could be found.
   How many polypeptide chains are in this protein? (Enter your answer as a whole number).



-Here is a hint that was given: The dinitrophenyl derivative identifies the end groups of each polypeptide chain. Start by calculating the number of moles of protein in the sample. Then calculate the number of moles of endgroup obtained.
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padrepadre
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9 years ago
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9 years ago
Another similar question, and the answer to it:

Quote
An oligomeric protein has a molecular weight of 126000. A 820 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger\'s reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 8.2 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the ?-amino groups of other amino acids could be found.

Answer:
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vcunningham22 Author
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#3
9 years ago
Perfect explanation-I was able to figure it out and get the correct answer. Thank you so much!
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#4
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9 years ago
Quote from: vcunningham22 (9 years ago)
Perfect explanation-I was able to figure it out and get the correct answer. Thank you so much!

Wonderful. Please mark which one you consider best as best answer.
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#5
3 years ago
Nice solution
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#6
2 years ago
thank you
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