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ehd123 ehd123
wrote...
Valued Member
Posts: 778
9 years ago
What are CFU assays and what are they used for?

Thank you =)
Read 1163 times
6 Replies
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience


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Replies
wrote...
Educator
9 years ago
CFU assays are performed to determine the hematopoietic growth potential of cells in the bone marrow, peripheral blood and cord blood.

http://books.google.ca/books?id=CvVEBAAAQBAJ&pg=PA45&lpg=PA45&dq=%22CFU+assays+are%22&source=bl&ots=71Y4B9eCfH&sig=l9ABpLfryC5p13HJ4Dsb9YUg2og&hl=en&sa=X&ei=tz5FVIWPJcmsyASY_IGgBg&ved=0CD8Q6AEwBQ#v=onepage&q&f=false

ehd123 Author
wrote...
Valued Member
9 years ago
Hmm, so if the cells to "test" generated a colony, that means that they have that potential? and if it generated a specific colony, say granulocyte monocyte, it has the potential to specifically make only monocytes?

Also, remember when I asked about the CD34/CD45 cells and what that meant? Is a CD34+/CD45- cells the same as a CD34+ cell?  Frowning Face
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience


wrote...
Educator
9 years ago
Is a CD34+/CD45- cells the same as a CD34+ cell?

You know what, that's a great question, but CD34+ doesn't ensure CD45-, so I'd say they are different.

I found this article that explains it well...

http://vethospital.tamu.edu/small-animal-hospital/orthopedics/stem-cell-therapy-faqs

In the laboratory setting several methods are used to characterize MSCs. A procedure called flow cytometry is often used to determine the presence or absence of a specific group of surface marker proteins (similar to a cell fingerprint) that are often present on MSCs (Figure 3A). Colony Forming Unit (CFU) assays determine the ability of individual cells to generate a new colony of cells (Figure 3B). Differentiation assays are used to determine the ability of MSCs to transform into cells that resemble bone, cartilage, and fat forming cells (Figure 4). Interestingly, some cells that aren’t considered stem cells share many of these same characteristics. For this reason, the true measure of MSCs that separates them from other cells is their ability to form new bone, cartilage, and fat tissue when transplanted to a distant location, not in a laboratory setting, but in a living patient. Restoration of bone, cartilage, and fat in the living setting provides definitive proof that an individual cell is a true stem cell (MSC).

ehd123 Author
wrote...
Valued Member
9 years ago
And can different media generate CD34+ to CD34+/CD45+?


So CFU assays are there to confirm  Slight Smile Thank you  Smiling Face with Halo
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience


wrote...
Staff Member
Educator
9 years ago
And can different media generate CD34+ to CD34+/CD45+?

Not generate, but select for.

Mastering in Nutritional Biology
Tralalalala Slight Smile
ehd123 Author
wrote...
Valued Member
9 years ago
Thank you for the clarification  Smiling Face with Halo
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience


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