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Emily52196 Emily52196
wrote...
Posts: 4
Rep: 0 0
9 years ago
Give the sequence and polarity of two DNA primers 6 nucleotides long that would allow you to amplify the entire fragment.

3’ CTACCGACTTCGTCCCATCGATGC 5’
5’ GATGGCTGAAGCAGGGTAGCTACG 3’
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rsb
wrote...
9 years ago
Here's a similar question. I hope it helps Undecided

Assume you want to use PCR to amplify the dashed gene XYZ region shown below. Note just one strand of a double stranded DNA molecule is shown. Design two primers to use in the PCR reaction. Write out the primer sequences, labeling the ends, 5' to 3' gene

XYZ 5'-TAGGCTAACGGCCATTACGCG-----------------------CGCGTTAAGCATGCTTAATG-3'

Primer 1
5'-TAGGCTAACGGCCATTACGCG-3'

Primer 2
5'-CATTAAGCATGCTTAAACGCG-3'
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