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Home Q & A Board Laboratory Help Referencing Help
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ehd123 ehd123
wrote...
Valued Member
Posts: 778
Rep: 54 2
9 years ago
Hi everyone Slight Smile

I am looking for a reliable and credible source to search for sequences of the mRNA of proteins.. I shall be screening for them, and need them to be reliable.. Any thoughts or advice please? Slight Smile

Have a good one,
and Many thanks in advance
Read 1233 times
11 Replies
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience

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Replies
wrote...
#1
Staff Member
9 years ago
So what you're looking for is a search where you type in the protein and it gives you the mRNA sequence?
- Master of Science in Biology
- Bachelor of Science
ehd123 Author
wrote...
#2
Valued Member
9 years ago
Precisely Slight Smile Did you happen to have come across such a database?
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience
wrote...
#3
Staff Member
9 years ago
I remember using BLAST as a university student.

http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastp&BLAST_PROGRAMS=blastp&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome

Have you tried their service?
- Master of Science in Biology
- Bachelor of Science
ehd123 Author
wrote...
#4
Valued Member
9 years ago
Not yet. This is my first time.. Will try looking right now
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience
wrote...
#5
Staff Member
9 years ago
https://www.youtube.com/watch?v=t8fKz9rvuOk&feature=youtu.be
- Master of Science in Biology
- Bachelor of Science
ehd123 Author
wrote...
#6
Valued Member
9 years ago
Hmm duddy, this is complicated  Face with Open Mouth enter accession number?
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience
wrote...
#7
Staff Member
9 years ago
Quote from: ehd123 (9 years ago)
Hmm duddy, this is complicated  Face with Open Mouth enter accession number?

It's what every scientist uses and is most reliable. I remember taking days to learn it, but once you do, it's powerful.

Tell me more about the protein you're searching.
- Master of Science in Biology
- Bachelor of Science
ehd123 Author
wrote...
#8
Valued Member
9 years ago
I am following the video you just posted.. I am looking for cytochromes.. There are many isoforms i would like to see if they are available in my mouse model..
But when I selected 'organism', there were numerous mouse models.. My mice are the NOD white mice.. I am lost Frowning Face
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience
wrote...
#9
Staff Member
9 years ago
These guys?

https://en.wikipedia.org/wiki/NOD_mice

Ones that have the diabetic trait
- Master of Science in Biology
- Bachelor of Science
ehd123 Author
wrote...
#10
Valued Member
9 years ago
Yep :/
B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience
wrote...
#11
Staff Member
9 years ago
Okay, according to one source, you must use Mice, Inbred NOD

See here: http://www.ncbi.nlm.nih.gov/mesh?Db=mesh&Cmd=DetailsSearch&Term=%22Mice,+Inbred+NOD%22%5BMeSH+Terms%5D
- Master of Science in Biology
- Bachelor of Science
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