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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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wywz2007 wywz2007
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11 years ago
I have a molecular biology question, can you help me to solve it?

You have cloned a cDNA molecule in the EcoRI and PstI sites of pBR322. Which bacterial strain (XLI-blue or JM105) are you going to use for the transformation experiment and briefly explain why?
(E. Coli  genotypes)
Xl1-blue lac- F’ [ lacIq lacZΔM15 Tn10(Tetr)]
JM105 lac- F’ [ proAB+ lacIq lacZΔM15]

thank you very much.
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wrote...
#1
11 years ago
Quote from: wywz2007 (11 years ago)
JM105 lac- F' [ proAB+ lacIq lacZΔM15]

This one is better because it is partly restriction-deficient and an overall good strain for cloning repetitive DNA.
wrote...
#2
11 years ago
use this as a guideline Slight Smile
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wywz2007 Author
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#3
11 years ago
thank you for your help.

i am a little bit confused with another question, could you explain to me? thank you very much

CTTCTCGCCATTG                       CGCTCAATTGCGC
GAAGAGCGGTAAC                    GCGAGTTAACGCG

Design two primers to clone this DNA fragment
   in the EcoR1 site of pUC18

                             AND
 Design two primers to clone this DNA fragment
      in the EcoRI and HindIII sites of pUC18

EcoRI = GAATTC      HindIII = AAGCTT


thanks again.
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