× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
e
5
e
4
4
d
4
o
3
p
3
t
3
3
m
3
p
3
m
3
f
3
New Topic  
selfstudy selfstudy
wrote...
Posts: 88
Rep: 0 0
11 years ago
It has been shown that the repair of UV induced dimers in a gene is coupled to transcription. Suggest a model for this and any experiments you would use to test your model.
Read 1500 times
2 Replies

Related Topics

Replies
wrote...
11 years ago
During transcription, the RNA polymerase has a repair mechanism that may be able to break up dimers as it runs across the DNA strand to replicate it.

One way to test this would be to do PCR on dna you know has been affected by UV as well as on unaffected DNA.

Once you have PCR products for both, you could get each of the products sequenced.

Once you have the sequences for both, you could compare the two and see if there have been any segments "cut out". If so, this theory may not be correct. However, if the two sequences are identical, you may be able to conclude that the DNA strand was repaired by the polymerase enzyme during replication and is now therefor identical to the unaffected DNA.
lcg347
selfstudy Author
wrote...
11 years ago
SO what would your model be?
New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  943 People Browsing
Related Images
  
 449
  
 357
  
 332
Your Opinion