× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
y
2
m
2
m
2
u
2
m
2
B
2
M
2
e
2
k
2
N
2
y
2
m
2
New Topic  
Asinense Asinense
wrote...
Posts: 20
Rep: 0 0
12 years ago
I am researching information for veterinarians on avian influenza and I am not clear on why you would perform an AGID instead of an ELISA.  I guess I do not really understand what the AGID test is and why it is optimal for this disease.
Hmmm, so why does the state lab run an AGID and rt-PCR instead of an ELISA and then a PCR?  Maybe ELISA can only test for any non-specific influenza A, anyone know?
Read 593 times
2 Replies

Related Topics

Replies
wrote...
12 years ago
The AGID test is an Agarose Gel Immunodiffusion assay. Basically, you put an antigen of interest (a virus) and a serum sample from an animal into adjoining wells of an agar plate. The antigen/antibody complexes that form (if any) should form a line between the two gels, basically solid precipitates in the agar. As far as I can tell, the specificity and sensitivity of this test (as you would expect, based on eye detection) is really low. ELISA analysis with an avian influenza virus antigen would be more accurate, if it is available.
wrote...
12 years ago
See links to support theory:

It appears that AGID can be run from blood, and is an indicator of exposure due to the presence of antibodies.  It does not indicate specific types of influenza.

"Rapid diagnosis can be made by the detection of viral antigen in tissue impression smears using immunofluorescence, or by antigen detection enzyme-linked immunosorbent assay (ELISA) on tissue homogenates. Pancreas and kidney are the organs in which antigen is most often demonstrable." (link)  

This suggests AGID is easier/ faster and perhaps more cost efective for testing large numbers of specimins with further testing only on those testing positive.
New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  601 People Browsing
Related Images
  
 1303
  
 347
  
 298
Your Opinion
Who's your favorite biologist?
Votes: 608