Urgent please help!!Genetics

You use PCR to amplify the regions you want.
You order restriction primers from a company (after designing them).
Once you have the primers you add the sample DNA, the 5' and 3' end primers, buffer, dNTPs, and polymerase.
You run the mixture in a thermocycler that repeatedly denatures, anneals, and extends the fragments of DNA.
Confirm the correct products through gel electrophorysis.

I would first use PCR (polymearse chain reaction) to amplify/increase the number of DNA copies I have. When I had a sufficient amount of DNA I could do use a technique called Compliment VNTR. (I am going to give you steps to forensic applications which is used to match a certain DNA genome with the perps DNA.)

1.PCR- makes more DNA
2.Endonuclease- cuts up the DNA
3.Electrophoresis- Organizes DNA using charges (large to small)
4.Southern Blot- transfer smear of DNA to membrane filter
5.Hybridize membrane with hot probe (The hot prob is the radioactive sequence of the certain sequence you want which will be complementary to the ones in the large samples of DNA)
6.Audoradiography- Compares the complementary sequences.

I hope this is what you were looking for or at least gives you some help.

I would first amplify the DNA for sequencing using PCR.

Once the sample is amplified, the DNA would need to be purified and the concentration measured.

Then I would set up a sequencing reaction using dideoxynuceotides which had been fluorescently labelled.

The sequence is then cleaned and run on an automated sequencer to get the sequence.

Process straightforward - search cycle sequencing for more details.