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toodaloo toodaloo
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10 years ago
4. The sequence below is present on one nucleotide chain of a DNA duplex that is opened up in a replication fork. Synthesis of a RNA primer begins on this template below at the base that is underlined.
3’..... GGTCATGGAATGGTTACCA...5’
a) If the RNA primer is six nucleotides long, what is its base sequence (written
5’ to 3’) (1 mark)?
5’-____________________________________

 b) Which enzyme synthesizes this RNA primer (1 mark)?
c) Which enzyme synthesizes DNA from the 3’-OH at the end of this primer (1 mark)?
d) Which enzyme degrades this primer and replaces it with DNA (1 mark)?
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10 years ago
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Synthesis of a RNA primer begins on this template below at the base that is underlined."
Which is the underlined base? Anyways, the 1st question is easy. The RNA is complementary to the DNA, so it goes 5->3 to the opposite direction of the DNA. You start from the underlined base, and you start putting complementary nucleotides (A-U, T-A, G-C, C-G).
For example IF THE UNDERLINED BASE WAS A :
3’..... GGTCATGGAATGGTTACCA ...5’
              5'-UACCUU-3' Leftwards Arrow that would be the RNA.


b) Primase
c) DNA polymerase.
d) DNA polymerase.
Note: If we talk about procaryotic DNA, you could replace questions c and d with DNA polymerase llI and DNA polymerase I respectively.
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