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ela ela
wrote...
11 years ago
You have found a yeast gene that appears to encode a DNA polymerase, and you hypothesize that a related gene exists in humans. Describe, in detail, the experiments you would use to test this hypothesis. Be as thorough as possible.
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wrote...
#1
11 years ago
the gene isolated has to be first sequenced ( automated sanger mathod). A BLAST of this sequence with human genome will predict the homology with any sequence in it. A high percent homology with small e-value will add-up to conclude a related gene in human
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#2
Donated
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11 years ago
You can test the ability for the DNA polymerase to be able to synthesize DNA under conditions where you replicate a human cell. Then run a gel. If the bands show up it means the DNA polymerase can work with humans, but if it doesn't show up it is likely that the same polymerase does not work in humans or does not exist there.

You can also sequence the polymerase and compare it to known human polymerases and see if any match.
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