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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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itzfinalflash itzfinalflash
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11 years ago Edited: 11 years ago, itzfinalflash
In the plasmid map for pUC19, it shows that the MCS is within the LacZ alpha gene, and the breakdown of X-gal is often used to identify successful DNA cloning. If the cells transformed from such a plasmid were of the E. Coli strain, then wouldn't the LacZ gene naturally found within the bacteria interfere with the results of the blue/white screening?
Post Merge: 11 years ago

Never mind; I'm an idiot. LacZ will only be expressed in E. Coli when allactose is present.
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Educator
11 years ago
Quote from: itzfinalflash (11 years ago)
Never mind; I'm an idiot. LacZ will only be expressed in E. Coli when allactose is present.

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