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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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Gini Gini
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You have isolated a plasmid from E. coli. You get the following results, using three restriction enzymes, 1 XhoI, 2 BamHI, and 3 EcoRI to perform six different digestions: single digests using each enzyme alone and double digests using each combination of two enzymes. 

enzyme# fragmentssizes (in kb)
XhoI110
BamHI23.75,  6.25
EcoRI24.25,  5.75
XhoI +  BamHI31.25,  3.75,  5
XhoI +  EcoRI32,  3.75,  4.25
BamHI + EcoRI41.25,  2.5,  3,  3.25

Draw a restriction map of the plasmid.
Textbook 
Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach


Edition: 3rd
Authors:
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SarahJosephSarahJoseph
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XhoI must cut the 10 kb plasmid only once, while BamHI and EcoRI each cut the plasmid at two sites. The XhoI site must cut the 6.25 kb BamHI fragment into 1.25 and 5 kb fragments, and not cut within the 3.75 kb fragment. The XhoI site must also cut the 5.75 kb EcoRI fragment into 2 and 3.75 kb fragments, and not cut within the 4.25 kb fragment. Finally, the digest with BamHI and EcoRI indicates that EcoRI must cut the 6.25 kb BamHI fragment into 3 and 3.25 kb fragments, as well as the 3.75 kb BamHI fragment into 1.25 and 2.5 kb.
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