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Home Q & A Board Biology-Related Homework Help Cell Biology
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Astatine Astatine
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9 years ago
You expect that a mutation in E. coli that was recently isolated results in loss of enzyme R activity. In looking for the gene, you find comparable sequences. Using an in vitro transcription translation system, you use the gene obtained from the mutant, as well as a wild type E. coli as a control. Following protein isolation and gel electrophoresis, you observe that the wild type E. coli produces a protein that is 35K in size, corresponding to enzyme R. However, the mutant strain produces a protein that is  25K in size. What does this information suggest? 
A) A nonsense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant. 
B) A large addition has occurred in the wild type strain, such that approximately 35 tandem repeats of this gene are present and are biologically active. 
C) A missense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.   
D) all of the above 
E) Not enough information is given.
Textbook 
Becker's World of the Cell

Becker's World of the Cell


Edition: 8th
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virionvirion
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Astatine Author
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#2
9 years ago
This is exactly what I needed, thank you.
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#3
9 years ago
Wonderful, don't forget to mark it solved Smiling Face with Open Mouth
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