× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
g
3
3
2
J
2
p
2
m
2
h
2
s
2
r
2
d
2
l
2
a
2
Home Q & A Board Biology-Related Homework Help Cell Biology
New Topic  
daveull daveull
wrote...
Posts: 23
Rep: 0 0
11 years ago
A 7kb was treated overnight with restriction endonuclease EcoRI. The separation of the digest on 0.08% anarose gel showed 3 bands of 1.5kb, 2.5 kb, and 3kb sizes.

How many restriction sites does this plasmid have?
Where on the plasmid are these sites located?
Read 1171 times
5 Replies

Related Topics

Replies
wrote...
#1
11 years ago
3 sites...
simple draw a circle and determine the sites each of size 1.5,2.5,3.5 total 7
wrote...
#2
11 years ago
There are three restriction sites.  I don't know if you have a diagram on your question but EcoR1 cuts at the palendromic site GAATTC.
wrote...
#3
11 years ago
Three cuts would give you 3 pieces that you see on the agarose gel.  You can't say where on the plasmid that the sites are located from the information given.  You can say that the size of the plasmid is 1.5 + 2.5 + 3 = 7 kb and that if one site is designated as zero, then another site is 3 kb away producing the large fragment, and another EcoRI site is either 2.5 kb or 1.5 kb from there towards the zero site.
wrote...
#4
11 years ago
None.  There is no such thing as an "anarose" gel.
wrote...
#5
11 years ago
There are two Eco RI sites which yields three plasmids.  The first site is 1.5 kB from the 5' end and the second site is 4 kB from the 5' end.  This yields three fragments of the size indicated.
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  1059 People Browsing
 114 Signed Up Today
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 34
  
 909
  
 256
Your Opinion
What percentage of nature vs. nurture dictates human intelligence?
Votes: 431
Previous poll results: Which industry do you think artificial intelligence (AI) will impact the most?