You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function ...

You should prepare 7
cuvettes with different NADH concentration. The first cuvette should function as a blank, i.e.
it should only include 0.1 M Tris-buffer (concentration = 0 mM). The last cuvette should include a NADH
concentration of 0.225 mM. You now need to work out the other 5 concentration that are
appropriate for the standard.

Material = 2 ml 4.5 mM NADH

The final volume in the cuvettes should always be 2 ml.

So cuvette 1 has the concentration 0 and cuvette 7 has the concentration 0.225. What concentration should the 5 other cuvettes have?