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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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Johnny4 Johnny4
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7 years ago
MacConkey plates and B-alactosidase assays are both ways to examine aspects of lactose metabolism. Breifly explain the differenc between them in terms of what they tell you about function. Why MacConkey plates rather than B-galactosidase assays are used as the inital screen for muants?
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7 years ago
You can use MacConkey agar to identify lac- mutants (cannot use sugar lactose for growth).

MacConkey agar contains other nutrients, so all cells can grow; but contains significant amount of lactose and pH indicator. If cell can use lactose, will produce acid (fermentation will occur even on colony when oxygen is exhausted during vigorous growth) and colony will turn red. If colony remains white, it is a lac mutant.
Source  http://www2.nau.edu/~fpm/bio205/lect15.html
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