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Home Q & A Board Biology-Related Homework Help Biochemistry
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rivers.butterfly rivers.butterfly
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11 years ago
For example we could use succinate dehydrogenase as a mitochondrial marker enzyme, after doing density gradient centrifugation. What other similar enzymes or molecules should we use in order to identify other cell organelles? Thank you!
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#1
11 years ago
you could use antibodies to do this.  Find a protein that localizes to a speciffic organelle, such as histones for the nucleus, a membrane receptor for the cell membrane, COX2 for the ER ect.  Then do a western blot on your extract.
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fireheart_girfireheart_gir
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#2
11 years ago
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