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Determination of Km value

spamlovesham

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12 years ago

Determination of Km value

I have constructed a Lineweaver-Burk plot from a table of results that I did in a lab experiment to try and determine the Km value of a certain enzyme. I have been trying to get the value of Km by taking the slope/y-intercept but that seems to give me a ridiculously wrong answer of 3.17mM. Km is supposed to be the substrate amount to produce half of vmax. The maximum amount of substrate added was 2.4mM. I get the feeling that I am completely screwing this up. All humility aside, can someone please point me in the right direction? (I hope the picture attaches)
TIA!!
-Sam
((The velocity is measured by absorbance))

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ziadelhussein

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12 years ago

I do not know about the the slope/y-intercept.
But -1/Km is the x-intercept in a Lineweaver-Burk plot.

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