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pado pado
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7 years ago
A particular restriction enzyme, such as EcoRI,
   
if used on both donor DNA and vector DNA, can allow those overhanging ends of the two different DNAs to match up and reanneal (or renature).

   
can be used to create recombinant DNA molecules.

   
cuts at random places in the DNA, creating new combinations and new genetic variation.

   
cuts at a palindromic sequence, leaving an "overhang" of 4-5 nucleotides.
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wrote...
7 years ago
if used on both donor DNA and vector DNA, can allow those overhanging ends of the two different DNAs to match up and reanneal (or renature).

   
can be used to create recombinant DNA molecules.

   
cuts at random places in the DNA, creating new combinations and new genetic variation.
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