Genetic and environmental interactions in ototoxic deafness

(a) III I 1 IV I O I 1 2 V I I 1 2 3 (b) l Streptomycin interacts with - foot of stem loop and interferes Mutations that disrupt streptomycin resistance. . with translation. .I II I. O O 1 2 3 I I I 2 3 I O I O 3 4 I I 4 5 6 7 8 9 3' end of E. coli 165 rRNA base pairing at the foot of the stem loop result in i l l O I I 4 5 I I O 4 5 6 I I I I O O I 5 6 7 8 9 O I O I 10 11 12 13 (C) 3' end of human mt 12$ rRNA 3 Human stem loop has a - more open foot than E. coli. Wild type Mutant --. all .1 4-1 A—'G —> I l Mutations that extend the base pairing at ~’ the foot of the stem loop of human 125 E rRNA result in aminoglycoside sensitivity. 1