Replica plating

(a) 1 457 1 324 57, my 9y Compare complete and minimal medium plates. Conclusion: colonies 1, 4, 5, 7, 9, and 10 are prototrophs, and colonies 2, 3, Re Iica late Complete medium A Minimal medium 6, and 8 are auxotrophs. I Replica plate (b) 1 ' 1 , 1 , 3 4 5 7 4 5 7- 3 2 4 5 7 6 9% Minimal plus alanine (Ala) Minimal plus proline (Pro) Compare to minimal medium plate. Conclusion: colony 3 is a/a’. Conclusion: colony 6 is pro: Compare to minimal medium plate. J (c) Replica plate from complete medium I Compare to minimal l medium plate. Conclusion: 1, 5, 7, and 9 are lad and colonies 4 and 10 are /aC‘. Auxotrophic colonies 2,3,6, and 8 do not grow without supplementation. 1, 57 9/‘ Lactose medium Comparing the results of the three supplemented minimal media to minimal medium identifies colony 8 as an auxotroph with an unknown genotype. 2 1 5 7,_ 6 9/ Lactose plus alanine and proline Minimal plus alanine and proline Compare to minimal medium plate. Conclusion: colony 2 is a/a: pro: x; Compare to minimal medium plus alanine and proline plate. Colony 2 is ala’, pro: lac‘; colony 3 is a/a', lac',‘ colony 6 is pro, lac. Comparing the results of the lactose-containing media to previous results identifies the prototrophic colonies 4 and 10 to be lac: