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Home Q & A Board Biology-Related Homework Help Cell Biology
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alenavar alenavar
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6 years ago
Which of the following statements about gel-transfer hybridization (or Southern blotting) is false?
 
  (a) This technique involves the transfer of DNA molecules from gel onto nitrocellulose paper or nylon paper.
  (b) In this technique, single-stranded DNA is separated by electrophoresis.
  (c) A labeled DNA probe binds to the DNA by hybridization.
  (d) The DNA that is separated on a gel is not labeled.
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#1
6 years ago
(b) During Southern blotting, double-stranded DNA is loaded onto the agarose gel. The DNA becomes denatured (and thus single-stranded) as it gets transferred by the alkali solution from the gel to the nitrocellulose or nylon sheet.
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