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toodaloo toodaloo
wrote...
Posts: 63
Rep: 6 0
10 years ago
Which of the following are potential restriction enzyme sites (circle candidates)(2 marks)?
a. 5’-aggcct b. 5’-ttataa c. 5’-gaccag d. 5’-ttaatt

which are the answer
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wrote...
Staff Member
A month ago
The potential restriction enzyme site candidates are 5’-gaccag (recognized by AciI) and 5’-ttaatt (no known recognition site). Circle these two candidates for your answer. 🧪🔬

Remember that the presence of a recognition site does not guarantee that the enzyme will cut at that location, as other factors (such as methylation) can influence enzyme activity.

5’-aggcct: This sequence does not match any known restriction enzyme recognition site.
5’-ttataa: This sequence does not match any known restriction enzyme recognition site.
5’-gaccag: This sequence contains the recognition site for the enzyme AciI (GACNNN/NNGTC).
5’-ttaatt: This sequence does not match any known restriction enzyme recognition site.
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