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Why are leading and lagging strand primers removed rather than joined with Okazaki fragments?

cmperez1988

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Why are leading and lagging strand primers removed rather than joined with Okazaki fragments?

Why are leading and lagging strand primers removed rather than joined with Okazaki fragments?

▸ The primers do not hydrogen bond to the template strand correctly.

▸ They have to be removed for leading and lagging strand synthesis to begin.

▸ Primers are too short to efficiently bind to Okazaki fragments.

▸ They contain nucleotides with 2'OH groups, and are targeted for excision by DNA Polymerase.

▸ They lack the 3' OH that would permit them to be covalently linked to the Okazaki fragments.

Textbook

Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach

Edition: 3^rd

Authors: Sanders, Bowman

Read 94 times

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