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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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AnePerrier AnePerrier
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2 years ago
Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s?

I.Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading 
frame, resulting in a fusion protein for each chain.
II.Disulfide bonds were induced to form between the cysteine residues of the purified insulin A 
and B chains.
III.Both human insulin genes were reverse translated to create synthetic genes.
IV.EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional 
cloning of each into a separate plasmid vector.


▸ I, II, III, IV

▸ III, IV, I, II

▸ IV, III, I, II

▸ III, I, II, IV

▸ I, III, IV, II
Textbook 
Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach


Edition: 3rd
Authors:
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vrundavrunda
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2 years ago
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AnePerrier Author
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2 years ago
Correct Slight Smile TY
deathDoUsPart
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Yesterday
Smart ... Thanks!
jodie1
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2 hours ago
Thanks for your help!!
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