Top Posters
Since Sunday
5
a
5
k
5
c
5
B
5
l
5
C
4
s
4
a
4
t
4
i
4
r
4
Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
New Topic  
AnePerrier AnePerrier
wrote...
Go to Answer
Posts: 106
Rep: 0 0
2 years ago
Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s?

I.Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading 
frame, resulting in a fusion protein for each chain.
II.Disulfide bonds were induced to form between the cysteine residues of the purified insulin A 
and B chains.
III.Both human insulin genes were reverse translated to create synthetic genes.
IV.EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional 
cloning of each into a separate plasmid vector.


▸ I, II, III, IV

▸ III, IV, I, II

▸ IV, III, I, II

▸ III, I, II, IV

▸ I, III, IV, II
Textbook 
Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach


Edition: 3rd
Authors:
Read 39 times
1 Reply
Replies
Answer verified by a subject expert
vrundavrunda
wrote...
#1
Posts: 84
Rep: 0 0
2 years ago
Sign in or Sign up in seconds to unlock everything for free
More solutions for this book are available here
1

Related Topics

AnePerrier Author
wrote...

2 years ago
Thanks
avance37
wrote...

Yesterday
Just got PERFECT on my quiz
smdavis
wrote...

2 hours ago
I appreciate what you did here, answered it right Smiling Face with Open Mouth
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  1339 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 379
  
 53
  
 41
Your Opinion
Do you believe in global warming?
Votes: 370
Previous poll results: What percentage of nature vs. nurture dictates human intelligence?