how to find hill nnumber and min of subunit

lisa142

wrote...

13 years ago

how to find hill nnumber and min of subunit

How do you find the hill number (nH) and minimum number of subunits in a hill plot graph? Can you give me like an example to show me it? Thnks!

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bio_man

wrote...

Educator

13 years ago

Hey, could you provide some sort of example of what you're referring to.

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duddy

wrote...

Staff Member

13 years ago

It's the slope...

In hill plots, the cooperative binding to multiple sites is nH < n. The graph shows Hill plots for the two examples below:

The noncooperative binding of ligand (blue) has a Hill coefficient, nH = 1.0.
The cooperative binding of ligand (red) has a Hill coefficient, nH = 1.8 (the maximum slope on the Hill plot).

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lisa142 Author

wrote...

13 years ago

For example, from the table,

I find p50 to be about 71 but what would the nH be? Please really show me how to get this. I'm stuck getting the fact nH would be the max slope. I keep getting different numbers for each of the points I try. Need to know this for my test....

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duddy

wrote...

Staff Member

13 years ago

Quote from: lisa142 (13 years ago)

I thought you simply use the formula (y2 - y1) / (x2 - x1) = the slope. Since it's a linear relationship, you shouldn't get much deviation from one point to the next.

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- Master of Science in Biology
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