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Home Q & A Board Biology-Related Homework Help Cell Biology
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trin trin
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12 years ago

What would happen if you forgot to add Gram's Iodine during the staining procedure?
Answer
      Gram positive would stain light purple or give a false G-.
      Gram negative would stain a light purple or give a false G+ result.
      Gram positive would stain a darker purple color.
      Decolorization step would not work.

D???
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#1
12 years ago
Iodine (Gram's iodine) solution acts as a mordant and fixes the dye (Crystal violet), making it more difficult to decolorize and reducing some of the variability of the test. So, without it the Crystal violet stain may decolorize. Meaning everything may look red due to the red stain (safranin or carbol fuchsin).
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trin Author
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12 years ago
So D?
Volunteers are seldom paid; not because they are worthless, but because they are PRICELESS!
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#3
12 years ago
Can't be d, that has to do with alcohol being added, still not 100% sure.
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