× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
A
6
j
6
c
5
m
5
C
5
d
5
s
5
n
4
i
4
d
4
d
4
J
4
New Topic  
selfstudy selfstudy
wrote...
Posts: 88
Rep: 0 0
11 years ago
You want to make a bunch of DNA from a sample you have, but you only want a certain sequence from the DNA you've isolated. What do you do? Give any components involved in the process, in addition to stating what the process is.
Read 2137 times
2 Replies

Related Topics

Replies
wrote...
11 years ago
But one thing proteins can't do is make copies of themselves. ... Some mutations have no effect, and others produce new versions of proteins that ... if you know the sequence of nucleotide building blocks on one strand, you know ... DNA copying process, which goes on much faster in rapidly dividing cancer
wrote...
Donated
Trusted Member
11 years ago
I would first use PCR (polymearse chain reaction) to amplify/increase the number of DNA copies I have. When I had a sufficient amount of DNA I could do use a technique called Compliment VNTR. (I am going to give you steps to forensic applications which is used to match a certain DNA genome with the perps DNA.)
1.PCR- makes more DNA
2.Endonuclease- cuts up the DNA
3.Electrophoresis- Organizes DNA using charges (large to small)
4.Southern Blot- transfer smear of DNA to membrane filter
5.Hybridize membrane with hot probe (The hot prob is the radioactive sequence of the certain sequence you want which will be complementary to the ones in the large samples of DNA)
6.Audoradiography- Compares the complementary sequences.
jh5891,  daparks27
New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  713 People Browsing
 113 Signed Up Today
Related Images
  
 72
  
 208
  
 289
Your Opinion
What percentage of nature vs. nurture dictates human intelligence?
Votes: 432