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Home Q & A Board Laboratory Help Upper-Year Courses
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adriata adriata
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7 years ago
I understand that diluting the ligation reaction mixture prior to transformation increases the transformation efficiency, but why is it that less DNA yields better results in this case?
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5seal5seal
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7 years ago
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#2
7 years ago
At high [DNA] you also could have an increased amount of 'impurities' (salts, etc) that can affect transformation efficiencies.
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