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Shotgun Sequencing

adrii01

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11 years ago

Shotgun Sequencing

In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.

What are the steps in the shotgun approach to whole-genome sequencing?

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padre

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11 years ago

1) multiple copies of the same chromosome are prepared

2) Chromosome copies are broken into 1-kb fragments

3) 1 kb fragments are cloned into plasmids

4) the plasmids are sequenced

5) A computer combines the fragment sequences There is no use for RNA (1-kb fragments are transcribed into RNA) in the Sanger method = full genome sequencing i.e. the shotgun approach.

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FusedMentality, navforever, Violet739, bio_man, mandm1445, khoapham12369

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queenhoa

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padre, krap, ballen123, sarah12J, jnilles2, Alaura93, kitchens, Ijit, jander, beansandrice, a000tt, bio_man, Oldy

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padre

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11 years ago

Thanks for posting the answer

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Mastering in Nutritional Biology
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ht716585

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11 years ago

Quote from: diana72g (11 years ago)

Multiple copies of the same chromosome are prepared
Chromosome copies are broken into 1-kb fragments
1-kb fragments are cloned into plasmids
The plasmids are sequenced
A computer combines the fragment sequences

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ponchoscowgirl

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11 years ago

great answer- thanks!

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kemmy100

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11 years ago

great answer

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teriingura

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11 years ago

thank you

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navforever

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11 years ago

thnk u

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snakedoc_5

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11 years ago

Thanks!

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sarah12J

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#10

11 years ago

great answer

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hherna09

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#11

11 years ago

thanks

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jpegueros

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#12

11 years ago

awesome thanx!

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rayoflght

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#13

11 years ago

Thank you!

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