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adrii01 adrii01
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11 years ago
In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.

What are the steps in the shotgun approach to whole-genome sequencing?

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#1
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11 years ago
1) multiple copies of the same chromosome are prepared

2) Chromosome copies are broken into 1-kb fragments

3) 1 kb fragments are cloned into plasmids

4) the plasmids are sequenced

5) A computer combines the fragment sequences There is no use for RNA (1-kb fragments are transcribed into RNA) in the Sanger method = full genome sequencing i.e. the shotgun approach.
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queenhoaqueenhoa
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11 years ago
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#3
Staff Member
Educator
11 years ago
Thanks for posting the answer
Mastering in Nutritional Biology
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#4
11 years ago
Quote from: diana72g (11 years ago)
  • Multiple copies of the same chromosome are prepared
  • Chromosome copies are broken into 1-kb fragments
  • 1-kb fragments are cloned into plasmids
  • The plasmids are sequenced
  • A computer combines the fragment sequences
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#5
11 years ago
great answer- thanks!
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#6
11 years ago
great answer
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#7
11 years ago
thank you
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#8
11 years ago
thnk u
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#9
11 years ago
Thanks!
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#10
11 years ago
great answer
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#11
11 years ago
thanks Slight Smile
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#12
11 years ago
awesome thanx!
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#13
11 years ago
Thank you!
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