Biochemistry

To express Tat Protein in CD4 T cells without pre-activation

To express Tat Protein in CD4 T cells without pre-activation

Hello All
My experiment plan: To express Tat protein in CD4 T cell without pre-activation. I have tried different vectors and virus such as MND-AAV6 EGFP, CMV-AAV6 EGFP, AdRGD EGFP, SFFV-Lentivirus EGFP, MSCV-Lentivirus EGFP , HIV-VSV-G-Luc and

Harry Tian   177   N/A

Harry Tian

Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojou

Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojou

The Nutrition Facts label for crackers states that 1 serving contains 19 g of carbohydrate, 4 g of fat, and 2 g of protein.

a.   Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojoules, for ea

bio_man   380   N/A

bio_man

Genetics/production/pedigree questions

Genetics/production/pedigree questions

1. A population is at genetic equilibrium if

Select one:
a. there is no change in population numbers over time
b. small populations migrate slowly
c. there is no change in allele frequencies over time
d. superior breedi

katybee9   297   N/A

katybee9

Active sodium ion pumping in a membrane

Active sodium ion pumping in a membrane

Hello

Assume I have a membrane of volume V, and potential Vm across it.Inside, there's some concentration of sodium Cna(in), chloride Ccl(in) and neutral macromolecule b (Cb).

Also, the sodium is being actively transported

M_M_S   390   N/A

M_M_S

You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function ...

You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function ...

You should prepare 7
cuvettes with different NADH concentration. The first cuvette should function as a blank, i.e.
it should only include 0.1 M Tris-buffer (concentration = 0 mM). The last cuvette should include a NADH
concentration o

SkövdeUni   357   N/A

SkövdeUni

Achieving the action potentials requirements for a neuron

Achieving the action potentials requirements for a neuron

The charge of a neuron cell when accumulating the charge differential for an electrical signal requires it to be relatively negative inside. The sodium ions collect inside via channels and the potassium ions exit the cell to approach the threshold. I

despues18   573   N/A

despues18

Enzymatic reaction and Q10

Enzymatic reaction and Q10

The book below (refering to enzymatic reactions)  says:
 "...cooling to 200K reduces its speed by six orders of magnitude.An intermediate with a lifetime of 1ms at room temperature may thus be stable for a few hours at 200K."

GPOWER   544   N/A

GPOWER

Based on the following ChIP-seq analysis, are set A and B genes transcribed?

Based on the following ChIP-seq analysis, are set A and B genes transcribed?

ChIP-seq analysis using an antibody against a
bromodomain protein on chromatin isolated from kidney glomerulus cells provides the following graph. The list on the left  provides names of the different genes being investigated. The green bar

biology1012   282   N/A

biology1012

DNA repair mutational analysis

DNA repair mutational analysis

A strain of E.coli contains a uvr mutations and thus has a lower % survival when exposed to increasing levels of UV. An alternate strain X contains an unknown mutation that has an even lower % survival (even more so than uvr mutation independently).

theworldman   343   N/A

theworldman

How to determinate the activity of peroxidase (HPR) in hemolymph of garden snail

How to determinate the activity of peroxidase (HPR) in hemolymph of garden snail

How to determinate the activity of peroxidase (HPR) in hemolymph of garden snail (Helix pomatia) ?

egzon123   1819   N/A

egzon123

Plasmid expresses truncated protein but original mutant gene doesn't. Explain?

Plasmid expresses truncated protein but original mutant gene doesn't. Explain?

A mutant mammalian gene has a premature stop codon but a Western blot detects no truncated protein. A plasmid is cloned from the wild-type gene and the mutant gene's premature stop codon is inserted at the same position. After transfecting t

DLS   1233   N/A

DLS

Calculate the ΔG'° and K'eq

Calculate the ΔG'° and K'eq

Fructose-6-phosphate + ATP  fructose-1,6-bisphosphate + ADP
Given the following data, calculate the ΔG'° and K'eq for the reaction. Show all mathematical work & indicate proper units.
R = 8.315 J/mol·K, T = 25°C,

wallrats   1552   N/A

wallrats

ELISA absorbency question answer checking please

ELISA absorbency question answer checking please

In each of the following circumstances indicate whether, for a sandwich assay, you would expect the final absorbance to be increased or decreased.
A)   Incomplete coating of well with antigen or antibody - decrease
B)   Incomplete blocking - i

DJC5492   1893   N/A

DJC5492

Calculating turnover number knowing the concentration of enzyme alone

Calculating turnover number knowing the concentration of enzyme alone

Could someone help me with this question ?

i got 3 /seconds!

 If 20.0 µg of hexokinase catalyses the phosphorylation of 155 mg of glucose in 1.93 min
 in 25.0 mL of a buffered solution at 37.0°C under saturating conditions,

Emma22   1947   N/A

Emma22

If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough

If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough

If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough to carry out work from a demanding job, what does this suggest about the reaction rates (are they fast or slow) of particular isoforms of the two enzy

Rich   1269   N/A

Rich

the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady sta

the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady sta

can somebody help with this question in attachment?
the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady state kinetic analysis yielded

sinch   4920   N/A

sinch

Molecular genetics. Explain the difference between the two mutation frequencies

Molecular genetics. Explain the difference between the two mutation frequencies

In yeast strain S288C (standard wild type) the spontaneous forward mutation frequency from tryptophan prototrophy (Trp+) to auxotrophy (Trp-) is 5 × 10^-7. For the tryptophan 5 gene (TRP5) the forward mutation frequency is 3 × 10^-8. Explain the diff

ashref   2234   N/A

ashref

guidance on how to approach this problem

guidance on how to approach this problem

2. You are given 180 mg of the following radioactively labeled carbohydrate, which contains a total of 3 μCi of 14C (14C = *). Draw the specific degradational pathway required to metabolize this molecule into a 3 Carbon subunit. Noting in this pathwa

charlane   1215   N/A

charlane

The polarity in a DNA strand

The polarity in a DNA strand

The polarity in a DNA strand is indicated by referring to one end as the 3' end and the other as the 5' end. Which structure is on the 3' end?

fibroin   586   N/A

fibroin

Write enzyme mechanisms for liver alcohol

Write enzyme mechanisms for liver alcohol

Write  enzyme  mechanisms  for
a.
Liver  alcohol  dehydrogenase,  which  interconverts  ethanol  and 
acetaldehyde  and  which  uses  NADH  as  a  co

sinch   1042   N/A

sinch

How to calculate amounts of acid and conjugate base?

How to calculate amounts of acid and conjugate base?

Hello,

I need help with my biochem lab. Here is the question...

"Prior to lab, use the Hendersen- Hasselbalch equation to calculate the amount of
Acetic acid and acetate needed to make a 100 mL solution of 50 mM
a

krustythe   865   N/A

krustythe

Why is the buffer region possible when we have ph=pka +/- 1

Why is the buffer region possible when we have ph=pka +/- 1

Find the [A-]/[HA] concentration ratio when ph=pka +/- 1. Why is the buffer region possible when we have ph=pka +/- 1 ??
helps please, thank you!

gh1991   863   N/A

gh1991

Alpha Chymotrypsin Appropriate Amino-Acids

Alpha Chymotrypsin Appropriate Amino-Acids

I'm having trouble understanding what exactly my practice test is asking.

What type of interaction would you expect between the CH2 groups of residue 4 of the dipeptide and the atoms of residue X? What interation is expected between

generalkorn12   1116   N/A

generalkorn12

Calculate the value of the ratio K2 / K1 of these binding constants

Calculate the value of the ratio K2 / K1 of these binding constants

A protein P has two binding sites for oxygen as shown:

         K1                 K2
      P + O2 ⇄ P O2            and             P O2 + O2 ⇄ P (O2)2

where the binding constan

xax   1074   N/A

xax

Which is not directly exposed to the stroma, but is exposed to the thylakoid lum

Which is not directly exposed to the stroma, but is exposed to the thylakoid lum

Which is not directly exposed to the stroma, but is exposed to the thylakoid lumen?
A) PSI.
B) PSII.
C) both PSI and PSII.
D) None of the above because both PSI and PSII are exposed to the stroma.

mikael   526   N/A

mikael

In the Z scheme describing electron flow in photosynthesis, the electron donor t

In the Z scheme describing electron flow in photosynthesis, the electron donor t

In the Z scheme describing electron flow in photosynthesis, the electron donor to P680 is ________, while the electron donor to P700 is ________.
A) H2O; plastocyanin
B) plastocyanin; H2O
C) O2; plastocyanin
D) plastocyanin; O

mikael   234   N/A

mikael

What is wavelength of light if the energy of one photon is 2.925 × 10-19 J? (h =

What is wavelength of light if the energy of one photon is 2.925 × 10-19 J? (h =

What is wavelength of light if the energy of one photon is 2.925 × 10-19 J? (h = 6.63 × 10-34 J s and c = 3.00 × 108 m s-1)
A) 680nm
B) 700 nm
C) 5.8 m
D) 1.5 × 10-3 nm

mikael   254   N/A

mikael

Plant pigments which can absorb light include

Plant pigments which can absorb light include

Plant pigments which can absorb light include
A) chlorophyll a.
B) chlorophyll b.
C) carotenoids and phycobilins.
D) All of the above.
E) A and B only.

mikael   470   N/A

mikael

Carotenoids, phycobilins, xanthophylls and chlorophyll molecules (that are not i

Carotenoids, phycobilins, xanthophylls and chlorophyll molecules (that are not i

Carotenoids, phycobilins, xanthophylls and chlorophyll molecules (that are not in the special pair) ________.
A) can transfer light energy to chlorophylls
B) can absorb light energy
C) may prevent the formation of reactive superoxide a

mikael   891   N/A

mikael

Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say

Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say

Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say that one dopamine molecule can activate 20 G-proteins. In the absence of Ca2+ entry, activated adenylyl cyclase synthesizes 200 cAMP molecules. Two cAMP molecules are req

hottcakez   690   N/A

hottcakez

Cellular Signaling. Help please!?

Cellular Signaling. Help please!?

Homework
Instructions: On the answer form (download from Learn) write your answers in the space provided. For each question write the answer in one complete sentence and explain/support your answer with one or more additional supporting sentenc

erind.guzman   1080   N/A

erind.guzman

Dopamine is a neurotransmitter that binds to a specific type of GPCR.

Dopamine is a neurotransmitter that binds to a specific type of GPCR.

Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say that one dopamine molecule can activate 20 G-proteins. In the absence of Ca2+ entry, activated adenylyl cyclase synthesizes 200 cAMP molecules. Two cAMP molecules are req

erind.guzman   1041   N/A

erind.guzman

Peptide Purity Grades-chineseprptide company

Peptide Purity Grades-chineseprptide company

 Peptide Purity Grades   
The Peptide Quality Standard
What peptide purity you need for your applications, we made it easy!
Purity of peptides is a critical factor that affects the success and meaningful outcome of a research project. Ma

chinesepeptide   615   N/A

chinesepeptide

Stapled Peptides-chinesepeptide

Stapled Peptides-chinesepeptide

The α-helix conformation being the most common element of protein secondary structures, has received considerable attention in drug discovery. High conformational instability of peptides leading to proteolytic cleavage and low bioavailability can be

chinesepeptide   941   N/A

chinesepeptide

Draw and label an action potential, in the form of a graph.

Draw and label an action potential, in the form of a graph.

Draw and label an action potential, in the form of a graph.

colleen   1141   N/A

colleen

Draw a graph showing change in membrane permeability (don't worry about includin

Draw a graph showing change in membrane permeability (don't worry about includin

Draw a graph showing change in membrane permeability (don't worry about including the units of permeability) to sodium and potassium during the course of an action potential. For reference, superimpose a graph of the action potential.

colleen   611   N/A

colleen

Draw graphs showing the effects of hypokalemia and hyperkalemia on action potent

Draw graphs showing the effects of hypokalemia and hyperkalemia on action potent

Draw graphs showing the effects of hypokalemia and hyperkalemia on action potential production. Don't worry about exact millivolt values - the point is to show that you understand the effects of these conditions relative to normal.

colleen   702   N/A

colleen

What is the condition under which an enzyme is defined as having a “ping-pong”

What is the condition under which an enzyme is defined as having a “ping-pong”

What is the condition under which an enzyme is defined as having a “ping-pong” mechanism?
A.  Enzyme (or enzyme/prosthetic group) is modified by substrate
B.  The enzyme has a dissociable coenzyme
C.  The first substrate

couple007   478   N/A

couple007

Which of the following single point mutations would abolish the capacity of

Which of the following single point mutations would abolish the capacity of

Which of the following single point mutations would abolish the capacity of E. coli to grow on several sugars other than glucose?
A.  an operator mutation
B.  a repressor mutation
C.  a mutation in the cyclic AMP binding

couple007   435   N/A

couple007

In bacterial mismatch repair the repair enzyme complex recognizes

In bacterial mismatch repair the repair enzyme complex recognizes

In bacterial mismatch repair the repair enzyme complex recognizes the parental strand since
A.  the parental strand has histones attached.
B.  the daughter strand is always in fragments.
C.  the parental strand contains

couple007   421   N/A

couple007