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DJC5492 DJC5492
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8 years ago
In each of the following circumstances indicate whether, for a sandwich assay, you would expect the final absorbance to be increased or decreased.
A)   Incomplete coating of well with antigen or antibody - decrease
B)   Incomplete blocking - increase
C)   Sample not added - decrease
D)   Antibody not added - decrease
E)   Incorrect antibody used - decrease
F)   Enzyme tag not added - decrease
G)   Substrate not added - decrease
H)   Stopping reagent not added - increase
I)   Incubation temperatures set too high (for example an ELISA validated for incubations at room temperature was run at: a) 37°C and b) 70°C - increase
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