Introductory Courses

How does salt affect the enzyme activity of catalase breaking down H2O2?

How does salt affect the enzyme activity of catalase breaking down H2O2?

I'm working on this lab but i cant figure out why the salt is causing the catalase to react slower with higher salt concentrations.  I understand that the protein is being denatured but why? what ions are interacting with each other to caus

Shammyyy68   2607   4 Replies

bio_man

How to identify which sweetener is which given unlabeled sweetener packages?

How to identify which sweetener is which given unlabeled sweetener packages?

'm provided with unlabeled packets of sweeteners. Any chemicals I need are provided. I have only a microscope, a pipettor, a balance, a spectrophotometer,a hot plate, and a water bath. I know what substances are being tested and their ingredient

arooj1a2b3c   1182   2 Replies

arooj1a2b3c

Difinition of physiology

Difinition of physiology

Physiology is the study of the normal functions of the organ systems.?

jhwz210001   797   2 Replies

bio_man

how to determine pka's from titration curve

how to determine pka's from titration curve

I am trying to finish my buffer lap report. Right now I'm looking at a titration curve for a triprotic acid (phosphate buffer).  It was titrated against NaOH. There are 3 curves on the graph so there are 3 pka's I need to determine. I

krustythe   1037   1 Reply

padre

The gasoline consumed by an automatic is a fossil fuel consisting skeleton

The gasoline consumed by an automatic is a fossil fuel consisting skeleton

I need help

nadia1228   1350   1 Reply

f_zah1

Cell culture

Cell culture

In all of the videos about cell cultures I've seen they always use cell that were already in a medium and transfer them to another medium.

My question is, how do you grow the first cells?

Thx a lot

fiol10   3075   4 Replies

fiol10

LAB

LAB

1,Lab Simulation: Urinalysis

Performing and writing lab reports is an integral part of the biology program. This next set of 15 questions will guide you in the development of lab techniques. You are not required to perform the actual lab,

KONJO369   2888   1 Reply

bellatora

Can someone check the geno/pheno table in my lab and tell me if I did it right?!

Can someone check the geno/pheno table in my lab and tell me if I did it right?!

Just need to make sure I filled it out or did it right, thank you!

The10thDoctor   10426   4 Replies

f_zah1

how to read graphs absorbance, transmittance, activity in a hydrolysis of starch

how to read graphs absorbance, transmittance, activity in a hydrolysis of starch

experiment: hydrolysis of starch with help of amylase

Hello everyone. I don't know how to study for the lab. My teacher is very confusing, I have a problem reading the graphs and understand the experiment . I really need a help.

mihaella   1076   3 Replies

duddy

Mitochondria Lab

Mitochondria Lab

Mechanism of action of cyanide:
Cyanide acts as an irreversible inhibitor of the electron transport chain; specifically it blocks cytochrome oxidase which catalyzes the transfer of electrons from cytochrome a3 of complex IV to molecular oxygen d

vicster   2163   1 Reply

Kenz22

What determines if something found in supernatant vs. in pellet?

What determines if something found in supernatant vs. in pellet?

So what determines, in centrifugation, if something is found in the pellet or the supernatant.

kittytrinh   1659   1 Reply

duddy

How many beads did you use?

How many beads did you use?

How many beads did you use?

fvsu16   1576   1 Reply

bio_man

Lab: Digestion of albumin with pepsin, source of error?

Lab: Digestion of albumin with pepsin, source of error?

Hello! So I did a lab on the digestion of albumin with pepsin to find out the reaction rate for pepsin (under different circumstances) by measuring the absorbance a couple of minutes after adding albumin to test tubes containing pepsin and other diff

ilkey   1738   1 Reply

duddy

Compare and contrast anticlines and synclines

Compare and contrast anticlines and synclines

Compare and contrast anticlines and synclines . 

Compare and contrast domes and basins . 

Compare and contrast anticlines and domes . 

stozki   2064   2 Replies

bio_man

How has the construction of artificial levees and dams on the Mississippi River

How has the construction of artificial levees and dams on the Mississippi River

How has the construction of artificial levees and dams on the Mississippi River and its tributaries contributed to a shrinking of the Mississippi's delta and extensive wetlands

stozki   1233   1 Reply

padre

Things in blueberry juice?

Things in blueberry juice?

I looked blueberry juice under a microscope and came across these. Could somebody identify what they are? Thanks.

micros   773   1 Reply

bio_man

Once you are viewing a specimen using the 40x objective, which of the following

Once you are viewing a specimen using the 40x objective, which of the following

Once you are viewing a specimen using the 40x objective, which of the following should you NOT do?
 A) use the fine focus knob
 B) use the coarse focus knob
 C) change the light setting
 D) carefully center the specimen before

helloducky   1265   3 Replies

bio_man

Salivary amylase is a digestive enzyme secreted in saliva that starts the digestion of complex

Salivary amylase is a digestive enzyme secreted in saliva that starts the digestion of complex

EXERCISE 2: INVESTIGATING SALIVARY AMYLASE

Salivary amylase is a digestive enzyme secreted in saliva that starts the digestion of complex carbohydrates into smaller polysaccharides. Pancreatic amylase further digests the polysaccharides i

MercedesB   1784   1 Reply

fluff-n-stuff

Why in carbohydrate digestion lab activity would you test a substrate for Starch & Sugar?

Why in carbohydrate digestion lab activity would you test a substrate for Starch & Sugar?

I'm sorry if this doesn't make sense but I really need some help on this.  In my A&P lab im having to answer questions on a carbohydrate digestion test tube test.  Why in carbohydrate digestion lab activity would you test a su

deannad17   851   1 Reply

padre

Basic SEM Protocol

Basic SEM Protocol

I've seen a lot of protocols for bacterial suspension sample preparation for SEM, but they vary in some way or another. What is a basic, simple procedure? How hard is it to prepare samples for SEM?

micro   550   1 Reply

csimon97

Right fish for dissection

Right fish for dissection

I and my friend will do fish dissection in our school. We have all dissecting instruments (eg. scalpels, forceps, anatomical tweezers), but we do not know exactly which fish species we will dissect.
The fish should be big, have digestive system

Osa   629   1 Reply

padre

Effects of Dark Chocolate on the Normal Oral Microbial Flora

Effects of Dark Chocolate on the Normal Oral Microbial Flora

We conducted a research regarding the effect of dark chocolate on the normal oral microbial flora. We had 6 set ups:

1. baseline for normal oral microbial flora ( no food consumption)
2. after food intake
3. 15 mins after dark c

pallakjj   1219   3 Replies

padre

Setting up a lab

Setting up a lab

Does setting up a microbiology lab require any form of license/permit (generally)? Also, do you need to be affiliated with an educational institution in order to submit a paper in an established article?

micro   854   2 Replies

shawn-dog

Can someone explain this enzyme prac to me?

Can someone explain this enzyme prac to me?

Hi,

We did an experiment in bio where we put 1.25g pieces of liver in 5 different test tubes, and then poured a solution on them.  The solution for the first test tube was 20% hydrogen peroxide, 40% hydrogen peroxide, 60%, 80% and the

WCH990   727   3 Replies

padre

Mitochondria Onlien Lab

Mitochondria Onlien Lab

The Catalytic Effect of Intermediates and Inhibitors on the Krebs Cycle

One of the important concepts established by many of Krebs' experiments was that the organic acids that are produced during the citric acid cycle can stimulate ox

vicster   2093   2 Replies

kmpoulsen07

Mitochondria Lab Online

Mitochondria Lab Online

Begin your first experiment by developing a hypothesis to predict what will happen to oxygen consumption in the reaction flask after the addition of pyruvate. Develop a second hypothesis to predict how oxygen consumption will change in the flask upon

vicster   4281   N/A

vicster

Bio Enzyme Lab Online

Bio Enzyme Lab Online

1.   Role of Acarbose as an Invertase Inhibitor

This experiment is designed to help you determine whether acarbose functions as a competitive or noncompetitive inhibitor of invertase.

Set up an experiment at 50° C, buffer pH 4.0,

vicster   2063   N/A

vicster

Bio Enzyme Lab online

Bio Enzyme Lab online

Set up an experiment at 50° C with a buffer pH of 4.0 and a [ S ] of 0.0 mM. Add enzyme. Find the slope of the line and record data for this first measurement. Continue to take measurements by carrying out separate experiments with increasing concent

vicster   2238   N/A

vicster

Measuring DNA Damage

Measuring DNA Damage

For eukaryotic cells the comet assay is very common but what about prokaryotes such as bacteria? How would one measure / detect DNA damage?

micro   1032   10 Replies

micro

DETERMINING THE CONCENTRATION OF PROTEIN

DETERMINING THE CONCENTRATION OF PROTEIN

First, you will make 10ml of 2mg/ml (or 2000μg /ml) of BSA (Label “UNDILUTED”).
a.   How much will you weigh out (in GRAMS) for 10mL of 2mg/ml? ____________
Second, you will make 10ml of 20μg/ml (Label “20”) in a glass test tube.
In the

hieunguyen288   889   1 Reply

zoeksyrianos

Calculating Concentration

Calculating Concentration

In the question, I need to calculate concentration of two solutions.
One with 30ml of 10M metal solution(Fe 2+) and 50ml of buffer.
 Another with 10ml of 10M metal soltion (Zn 2+), 20ml of water, and 50ml of buffer.

I need to ca

kayleigh   646   1 Reply

zoeksyrianos

Please help with Hardy Weinberg question?

Please help with Hardy Weinberg question?

Hello,
I have a question about a Hardy Weinberg calculation but wanted to confirm if my approach is correct w/ the calculation of actual frequencies.
"...12 out of the 525 total duck population is showing signs of disease as result o

Jaimie   651   1 Reply

padre

How does magnesium sulfate harm the environment?

How does magnesium sulfate harm the environment?

I am doing a project in school about magnesium sulfate and the effect it has on the germination of mustard seeds. We have done our experiment and found out that magnesium sulfate does affect the germination of mustard seeds. So it can cause harm to t

fullforceg   684   1 Reply

Nikkyne

How do I analyze my standard curve if my data is off?

How do I analyze my standard curve if my data is off?

Hi,

I am having trouble doing my assignment because of bad results.

I created a standard curve for protein concentration from BSA. Then I did four serial dilutions of an unknown sample. Something went wrong while doing the dilu

Chordate   1110   5 Replies

barry

Fluorescence microscopy question?

Fluorescence microscopy question?

Fluorescence microscopy can be used with fluorescent stains to view structures within cells whose size is much less than Abbe?s resolution limit for light microscopy.  Explain how that is possible.

nurnur   1962   2 Replies

runit

Mendel's Law of Segregation

Mendel's Law of Segregation

Using the Punnett square to make predictions

Use the completed Punnett square in Part B to answer the questions below about the F2 generation.

Answer each question in the table by dragging the correct label to the appropriate lo

miavoo   1741   2 Replies

padre

Need help with lab report questions

Need help with lab report questions

Reaction of zinc metal and tincture of iodine.
Rewrite the overall reaction: a)Zn (s) + I_2 (alcohol) -> Zn^2+ 2I-
What is the oxidation half-reaction? b)_____________________________
What is the reduction half-reaction? c)__

gamingwings   6593   2 Replies

padre

What temperature kills a lettuce seed ?

What temperature kills a lettuce seed ?

Im doing a science project on " how does temperature affect the germination of lettuce seeds ? " and im planning to microwave the seeds / put it out in the sun and put it in the freezer / cooler , but i need to know how hot and cold the lettuce seed

Leeta c   1500   2 Replies

Alexx

How to control temperature in an experiment?

How to control temperature in an experiment?

For a science experiment I am testing how different temperature affect the germination of seeds. This experiment will span over a period of days to weeks, and I will need to keep the seeds at a constant temperature the entire time.

I want to have on

Ddn268   512   1 Reply

zme_9325

What is does control group and experiemtal group in the experimental design mean?

What is does control group and experiemtal group in the experimental design mean?

In this lab that I am doing I have to observe the effect of pH on the germination of radish seeds. I will grow some seeds with asprin, some with baking soda, and some in pure water. I will let the seeds germinate for a couple of days.

What is the C

franklynturtle   542   1 Reply

LIGHT