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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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mki mki
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9 years ago Edited: 9 years ago, mki
You decide to perform dideoxy sequencing on a PCR product. You add the appropriate 32P-labeled primer, DNA polymerase, DNA template (the PCR product), buffer, dNTP mix, and a small amount of one of the four ddNTPs to four reaction tubes. You run the reactions in the thermal cycler, load each reaction into a separate lane of a polyacrylamide gel, and separate the products by gel electrophoresis. In the figure below, the lanes are labeled according to the ddNTP added.
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Educator
9 years ago
Hello, see if this helps.
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Sel2
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A year ago
nice
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