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Home Q & A Board Laboratory Help Upper-Year Courses
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nursing nursing
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11 years ago
How quickly can they be detected in rabbits (how many test bleeds and how often?)
Do you remove the serum first and use column chromatography?
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11 years ago
The ELISA test.  You take the antigen in a buffered solution, and stick it to a plastic plate, then you put dilutions of the rabbit serum onto the plate.  If the antibodies stick, then they were specific to the antigen and you can detect them via an ezyme that sticks to the antibodies.  It's a multi-step process, takes a couple days.  Only one bleed is required, though you usually do a pre and post after 2, 4, 6 weeks, etc.
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