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Yeast2meetU Yeast2meetU
wrote...
Posts: 5
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8 years ago
Would it be possible to single individual yeast cells at home without the need for a micromanipulator? what is the cost of the cheapest micromanipulator if this isn't possible?

/pubmed/13666896 i found this, which may supply the answer but I couldn't find access to the text (also couldnt link the website due to account status).

Thanks!
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wrote...
#1
Educator
8 years ago Edited: 8 years ago, bio_man
It is possible:

http://www.ncbi.nlm.nih.gov/pubmed/8368006

http://onlinelibrary.wiley.com/doi/10.1002/yea.320090706/abstract

Why would you want to isolate a single cell? (I'm curious lol)
wrote...
#2
8 years ago
Yes would be possible
wrote...
#3
Educator
8 years ago
Quote from: Ax345 (8 years ago)
Yes would be possible

Care to elaborate?
Yeast2meetU Author
wrote...
#4
8 years ago
I would like to test the effect of different amino acids on individual cells to see their longevity and also to see the cell culture growth rate, i was hoping of some dye i could i use to be able to locate a cell from a crowd, i take it that may not be possible?
wrote...
#5
8 years ago
This sort of thing is rarely done on a single cell.

If you want to study growth, use selective growth medium.

I have no clue how to isolate a single cell, never needed it.
When you like a flower, you just pluck it out.
But when you love a flower, you water it daily.
The one who understands this, understands life.

- That's the difference between I like you and I love you.
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