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To express Tat Protein in CD4 T cells without pre-activation
Hello All My experiment plan: To express Tat protein in CD4 T cell without pre-activation. I have tried different vectors and virus such as MND-AAV6 EGFP, CMV-AAV6 EGFP, AdRGD EGFP, SFFV-Lentivirus EGFP, MSCV-Lentivirus EGFP , HIV-VSV-G-Luc and mRNA Tat, etc. But all vectors and viruses I tried didn't work Please give me some input Thanks Harry
Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojou
The Nutrition Facts label for crackers states that 1 serving contains 19 g of carbohydrate, 4 g of fat, and 2 g of protein.
a. Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojoules, for each food type. Round off the kilojoules for each food type to the tens place.
Select one: a. there is no change in population numbers over time b. small populations migrate slowly c. there is no change in allele frequencies over time d. superior breeding mates are selected
2. A farmer planted some bean seeds. When the seeds germinated, 209 of the seedlings were albino, a re
Assume I have a membrane of volume V, and potential Vm across it.Inside, there's some concentration of sodium Cna(in), chloride Ccl(in) and neutral macromolecule b (Cb).
Also, the sodium is being actively transported from the inside to the outside with Jna_a the active current density (constant). The outside has also some concentration of sodium Cna(
You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function ...
You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function as a blank, i.e. it should only include 0.1 M Tris-buffer (concentration = 0 mM). The last cuvette should include a NADH concentration of 0.225 mM. You now need to work out the other 5 concentration that are appropriate for the standard.
Achieving the action potentials requirements for a neuron
The charge of a neuron cell when accumulating the charge differential for an electrical signal requires it to be relatively negative inside. The sodium ions collect inside via channels and the potassium ions exit the cell to approach the threshold. Is it required to have this arrangement of sodium ion concentration to increase inside and potassium's outside, or would it be less
The book below (refering to enzymatic reactions) says: "...cooling to 200K reduces its speed by six orders of magnitude.An intermediate with a lifetime of 1ms at room temperature may thus be stable for a few hours at 200K."
Based on the following ChIP-seq analysis, are set A and B genes transcribed?
ChIP-seq analysis using an antibody against a bromodomain protein on chromatin isolated from kidney glomerulus cells provides the following graph. The list on the left provides names of the different genes being investigated. The green bars represent to extent to which the DNA sequences corresponding to gene promoters are recovered in the co-immunoprecipitation assay. Gen
A strain of E.coli contains a uvr mutations and thus has a lower % survival when exposed to increasing levels of UV. An alternate strain X contains an unknown mutation that has an even lower % survival (even more so than uvr mutation independently). However when these two are put together they have much lower % survival. Experiements showed that mutating more uvr components had no e
Plasmid expresses truncated protein but original mutant gene doesn't. Explain?
A mutant mammalian gene has a premature stop codon but a Western blot detects no truncated protein. A plasmid is cloned from the wild-type gene and the mutant gene's premature stop codon is inserted at the same position. After transfecting the mutant plasmid into the mutant gene, the Western blot now detects the truncated protein. Why?
Fructose-6-phosphate + ATP fructose-1,6-bisphosphate + ADP Given the following data, calculate the ΔG'° and K'eq for the reaction. Show all mathematical work & indicate proper units. R = 8.315 J/mol·K, T = 25°C,
ATP ADP + Pi [ΔG'° = -30.5 kJ/mol]
Fructose 1,6-bisphosphate Fructose 6-phosphate + Pi [ΔG'° = -16.0
In each of the following circumstances indicate whether, for a sandwich assay, you would expect the final absorbance to be increased or decreased. A) Incomplete coating of well with antigen or antibody - decrease B) Incomplete blocking - increase C) Sample not added - decrease D) Antibody not added - decrease E) Incorrect antibody used - decrease F) E
Calculating turnover number knowing the concentration of enzyme alone
Could someone help me with this question ?
i got 3 /seconds!
If 20.0 µg of hexokinase catalyses the phosphorylation of 155 mg of glucose in 1.93 min in 25.0 mL of a buffered solution at 37.0°C under saturating conditions, and in the presence of excess Mg2+ and ATP, calculate the turnover number of the mutant in s-1.
If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough
If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough to carry out work from a demanding job, what does this suggest about the reaction rates (are they fast or slow) of particular isoforms of the two enzymes alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) that would be present in the body? Explain your reasoning
the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady sta
can somebody help with this question in attachment? the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady state kinetic analysis yielded
Molecular genetics. Explain the difference between the two mutation frequencies
In yeast strain S288C (standard wild type) the spontaneous forward mutation frequency from tryptophan prototrophy (Trp+) to auxotrophy (Trp-) is 5 × 10^-7. For the tryptophan 5 gene (TRP5) the forward mutation frequency is 3 × 10^-8. Explain the difference between the two mutation frequencies.
2. You are given 180 mg of the following radioactively labeled carbohydrate, which contains a total of 3 μCi of 14C (14C = *). Draw the specific degradational pathway required to metabolize this molecule into a 3 Carbon subunit. Noting in this pathway we also incubated with a triose phosphate isomerase inhibitor (assume 100% inhibition). Include all intermediates, products, enzymes
Write enzyme mechanisms for a. Liver alcohol dehydrogenase, which interconverts ethanol and acetaldehyde and which uses NADH as a coenzyme. b. Tyrosine racemase, an enzyme that interconverts L-tyrosine and D-t
How to calculate amounts of acid and conjugate base?
Hello,
I need help with my biochem lab. Here is the question...
"Prior to lab, use the Hendersen- Hasselbalch equation to calculate the amount of Acetic acid and acetate needed to make a 100 mL solution of 50 mM acetate/acetic acid with a pH of 4.0. For this part 0.2 M acetic acid will be the stock solution. Measure out the correct amounts o
I'm having trouble understanding what exactly my practice test is asking.
What type of interaction would you expect between the CH2 groups of residue 4 of the dipeptide and the atoms of residue X? What interation is expected between NH3+ group of residue 4 and atoms of residue Y? Give an example of an amino acid residue expected for residue X and Y. Justify your cho
Which is not directly exposed to the stroma, but is exposed to the thylakoid lum
Which is not directly exposed to the stroma, but is exposed to the thylakoid lumen? A) PSI. B) PSII. C) both PSI and PSII. D) None of the above because both PSI and PSII are exposed to the stroma.
In the Z scheme describing electron flow in photosynthesis, the electron donor t
In the Z scheme describing electron flow in photosynthesis, the electron donor to P680 is ________, while the electron donor to P700 is ________. A) H2O; plastocyanin B) plastocyanin; H2O C) O2; plastocyanin D) plastocyanin; O2 E) O2; H2O
What is wavelength of light if the energy of one photon is 2.925 × 10-19 J? (h =
What is wavelength of light if the energy of one photon is 2.925 × 10-19 J? (h = 6.63 × 10-34 J s and c = 3.00 × 108 m s-1) A) 680nm B) 700 nm C) 5.8 m D) 1.5 × 10-3 nm
Plant pigments which can absorb light include A) chlorophyll a. B) chlorophyll b. C) carotenoids and phycobilins. D) All of the above. E) A and B only.
Carotenoids, phycobilins, xanthophylls and chlorophyll molecules (that are not i
Carotenoids, phycobilins, xanthophylls and chlorophyll molecules (that are not in the special pair) ________. A) can transfer light energy to chlorophylls B) can absorb light energy C) may prevent the formation of reactive superoxide anion D) A, B and C E) A and B only
Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say
Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say that one dopamine molecule can activate 20 G-proteins. In the absence of Ca2+ entry, activated adenylyl cyclase synthesizes 200 cAMP molecules. Two cAMP molecules are required to activate PKA and each molecule of PKA can phosphorylate 200 proteins. If 8 dopamine molecules bind to receptors, how many prot
Homework Instructions: On the answer form (download from Learn) write your answers in the space provided. For each question write the answer in one complete sentence and explain/support your answer with one or more additional supporting sentences.
1. Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say that one dopamine molecule can
Dopamine is a neurotransmitter that binds to a specific type of GPCR.
Dopamine is a neurotransmitter that binds to a specific type of GPCR. Let’s say that one dopamine molecule can activate 20 G-proteins. In the absence of Ca2+ entry, activated adenylyl cyclase synthesizes 200 cAMP molecules. Two cAMP molecules are required to activate PKA and each molecule of PKA can phosphorylate 200 proteins. If 8 dopamine molecules bind to receptors, how man
Peptide Purity Grades The Peptide Quality Standard What peptide purity you need for your applications, we made it easy! Purity of peptides is a critical factor that affects the success and meaningful outcome of a research project. Making a right choice of peptide purity is important while synthesizing a peptide for your applications. This will save time as well as c
The α-helix conformation being the most common element of protein secondary structures, has received considerable attention in drug discovery. High conformational instability of peptides leading to proteolytic cleavage and low bioavailability can be tackled via conformational stabilization of α-helical structure. Hydrocarbon stapled peptides are mini-proteins locked into their bioac
Draw a graph showing change in membrane permeability (don't worry about includin
Draw a graph showing change in membrane permeability (don't worry about including the units of permeability) to sodium and potassium during the course of an action potential. For reference, superimpose a graph of the action potential.
Draw graphs showing the effects of hypokalemia and hyperkalemia on action potent
Draw graphs showing the effects of hypokalemia and hyperkalemia on action potential production. Don't worry about exact millivolt values - the point is to show that you understand the effects of these conditions relative to normal.
What is the condition under which an enzyme is defined as having a “ping-pong”
What is the condition under which an enzyme is defined as having a “ping-pong” mechanism? A. Enzyme (or enzyme/prosthetic group) is modified by substrate B. The enzyme has a dissociable coenzyme C. The first substrate on and the last product off show competitive binding D. The reaction is bimolecular E. The two substrates bind i
Which of the following single point mutations would abolish the capacity of
Which of the following single point mutations would abolish the capacity of E. coli to grow on several sugars other than glucose? A. an operator mutation B. a repressor mutation C. a mutation in the cyclic AMP binding protein D. a mutation in glutamine synthetase E. C and D
In bacterial mismatch repair the repair enzyme complex recognizes
In bacterial mismatch repair the repair enzyme complex recognizes the parental strand since A. the parental strand has histones attached. B. the daughter strand is always in fragments. C. the parental strand contains more thymines. D. the parental DNA is methylated. E. the repair complex cannot tell the difference.
ADP + Pi [ΔG'° = -30.5 kJ/mol]
