Biochemistry

guidance on how to approach this problem

guidance on how to approach this problem

2. You are given 180 mg of the following radioactively labeled carbohydrate, which contains a total of 3 μCi of 14C (14C = *). Draw the specific degradational pathway required to metabolize this molecule into a 3 Carbon subunit. Noting in this pathwa

charlane   1215   N/A

charlane

Molecular genetics. Explain the difference between the two mutation frequencies

Molecular genetics. Explain the difference between the two mutation frequencies

In yeast strain S288C (standard wild type) the spontaneous forward mutation frequency from tryptophan prototrophy (Trp+) to auxotrophy (Trp-) is 5 × 10^-7. For the tryptophan 5 gene (TRP5) the forward mutation frequency is 3 × 10^-8. Explain the diff

ashref   2234   N/A

ashref

the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady sta

the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady sta

can somebody help with this question in attachment?
the muscle isoenzyme of lactate dehydrogenase is inhibited by lactate steady state kinetic analysis yielded

sinch   4920   N/A

sinch

If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough

If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough

If a person drinks regular large amounts of alcohol but is able to recover quickly and sober enough to carry out work from a demanding job, what does this suggest about the reaction rates (are they fast or slow) of particular isoforms of the two enzy

Rich   1269   N/A

Rich

Calculating turnover number knowing the concentration of enzyme alone

Calculating turnover number knowing the concentration of enzyme alone

Could someone help me with this question ?

i got 3 /seconds!

 If 20.0 µg of hexokinase catalyses the phosphorylation of 155 mg of glucose in 1.93 min
 in 25.0 mL of a buffered solution at 37.0°C under saturating conditions,

Emma22   1947   N/A

Emma22

ELISA absorbency question answer checking please

ELISA absorbency question answer checking please

In each of the following circumstances indicate whether, for a sandwich assay, you would expect the final absorbance to be increased or decreased.
A)   Incomplete coating of well with antigen or antibody - decrease
B)   Incomplete blocking - i

DJC5492   1893   N/A

DJC5492

Calculate the ΔG'° and K'eq

Calculate the ΔG'° and K'eq

Fructose-6-phosphate + ATP  fructose-1,6-bisphosphate + ADP
Given the following data, calculate the ΔG'° and K'eq for the reaction. Show all mathematical work & indicate proper units.
R = 8.315 J/mol·K, T = 25°C,

wallrats   1552   N/A

wallrats

Plasmid expresses truncated protein but original mutant gene doesn't. Explain?

Plasmid expresses truncated protein but original mutant gene doesn't. Explain?

A mutant mammalian gene has a premature stop codon but a Western blot detects no truncated protein. A plasmid is cloned from the wild-type gene and the mutant gene's premature stop codon is inserted at the same position. After transfecting t

DLS   1234   N/A

DLS

How to determinate the activity of peroxidase (HPR) in hemolymph of garden snail

How to determinate the activity of peroxidase (HPR) in hemolymph of garden snail

How to determinate the activity of peroxidase (HPR) in hemolymph of garden snail (Helix pomatia) ?

egzon123   1819   N/A

egzon123

DNA repair mutational analysis

DNA repair mutational analysis

A strain of E.coli contains a uvr mutations and thus has a lower % survival when exposed to increasing levels of UV. An alternate strain X contains an unknown mutation that has an even lower % survival (even more so than uvr mutation independently).

theworldman   343   N/A

theworldman

Based on the following ChIP-seq analysis, are set A and B genes transcribed?

Based on the following ChIP-seq analysis, are set A and B genes transcribed?

ChIP-seq analysis using an antibody against a
bromodomain protein on chromatin isolated from kidney glomerulus cells provides the following graph. The list on the left  provides names of the different genes being investigated. The green bar

biology1012   282   N/A

biology1012

Enzymatic reaction and Q10

Enzymatic reaction and Q10

The book below (refering to enzymatic reactions)  says:
 "...cooling to 200K reduces its speed by six orders of magnitude.An intermediate with a lifetime of 1ms at room temperature may thus be stable for a few hours at 200K."

GPOWER   544   N/A

GPOWER

Achieving the action potentials requirements for a neuron

Achieving the action potentials requirements for a neuron

The charge of a neuron cell when accumulating the charge differential for an electrical signal requires it to be relatively negative inside. The sodium ions collect inside via channels and the potassium ions exit the cell to approach the threshold. I

despues18   573   N/A

despues18

You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function ...

You should prepare 7 cuvettes with different NADH concentration. The first cuvette should function ...

You should prepare 7
cuvettes with different NADH concentration. The first cuvette should function as a blank, i.e.
it should only include 0.1 M Tris-buffer (concentration = 0 mM). The last cuvette should include a NADH
concentration o

SkövdeUni   357   N/A

SkövdeUni

Active sodium ion pumping in a membrane

Active sodium ion pumping in a membrane

Hello

Assume I have a membrane of volume V, and potential Vm across it.Inside, there's some concentration of sodium Cna(in), chloride Ccl(in) and neutral macromolecule b (Cb).

Also, the sodium is being actively transported

M_M_S   390   N/A

M_M_S

Genetics/production/pedigree questions

Genetics/production/pedigree questions

1. A population is at genetic equilibrium if

Select one:
a. there is no change in population numbers over time
b. small populations migrate slowly
c. there is no change in allele frequencies over time
d. superior breedi

katybee9   297   N/A

katybee9

Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojou

Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojou

The Nutrition Facts label for crackers states that 1 serving contains 19 g of carbohydrate, 4 g of fat, and 2 g of protein.

a.   Using the nutrition values for one serving of crackers found above, calculate the energy, in kilojoules, for ea

bio_man   380   N/A

bio_man

To express Tat Protein in CD4 T cells without pre-activation

To express Tat Protein in CD4 T cells without pre-activation

Hello All
My experiment plan: To express Tat protein in CD4 T cell without pre-activation. I have tried different vectors and virus such as MND-AAV6 EGFP, CMV-AAV6 EGFP, AdRGD EGFP, SFFV-Lentivirus EGFP, MSCV-Lentivirus EGFP , HIV-VSV-G-Luc and

Harry Tian   177   N/A

Harry Tian

How many gene products are required to form this structure?

How many gene products are required to form this structure?

A cellular structure is composed of 20 proteins that have identical structures. How many gene products are required to form this structure?

Susan   1888   1 Reply

duddy

Amino Acid Polypeptide Q

Amino Acid Polypeptide Q

I need help on the review question:

How many molecules of water are used up in the breakdown of a polypeptide 15 amino acids in length?

B

barry   6074   1 Reply

bio_man

Amino acids phosphorylation

Amino acids phosphorylation

Which of the following amino acids could be a target for phosphorylation, and why?

The options are:

Phe, Tyr, Ala, Asp, Ser, Cys, Thr.

jonas   1445   1 Reply

bio_man

functional groups

functional groups

In the following table, indicate whether each functional group is polar or nonpolar and hydrophobic or hydrophilic. Which of these functional groups are found in proteins and lipids?

Functional Group

OH

- CH2

levik24   19254   1 Reply

bio_man

You are working in a research lab investigating lignin degradation by the bacterium Eatusdetree ...

You are working in a research lab investigating lignin degradation by the bacterium Eatusdetree ...

You are working in a research lab investigating lignin degradation by the bacterium Eatusdetree totalii which was isolated from a rotting piece of birch your supervisor found while strolling in the forest. The genomic sequence of this bacterium has n

star   1259   1 Reply

bio_man

Diffrence between allosteric inhibitor & feedback inhibition?

Diffrence between allosteric inhibitor & feedback inhibition?

what is the difference between allosteric inhibitor & feedback inhibition?...

Morbesety   9857   1 Reply

bio_man

A ribbon representation of a protein domain

A ribbon representation of a protein domain

Shown below is a ribbon representation of a protein domain. Name the recognizable secondary structures a, b, (c & d). Are c and d parallel or antiparallel?

minjai0322   1089   1 Reply

bio_man

membrane transport

membrane transport

1) Arrange the following in order of the greatest to least contribution to the ability of fatty acids to pack together.

 
 1. Short unsaturated tail
 2. Short saturated tail
 3. Long unsaturated tail
 4. Long saturated

chels194   1040   1 Reply

bio_man

What do cyanophages do to dead photosynthetic organisms?

What do cyanophages do to dead photosynthetic organisms?

What do cyanophages do to dead photosynthetic organisms? How do they do it? Why are they called 'cyanophages'?

short and sweet please 

Morbesety   1055   1 Reply

bio_man

What are intercellular junctions and why are they important?

What are intercellular junctions and why are they important?

What are intercellular junctions and why are they important? also

Contrast plasmodesmata, tight junctions, desmosomes, and gap junctions.

thanks!~

Morbesety   14605   1 Reply

bio_man

Multiple enzyme reactions

Multiple enzyme reactions

Many compounds are used in multiple enzyme reactions in the cell as a co-substrate. For example ATP and NADH. In a way those enzymes which need those substrates are competing for the shared substrate. Explain how cells may have developed a strategy t

minjai0322   1163   1 Reply

bio_man

In one tracer experiment glucose is labeled with C13 at its C1 [the carbon one, (which carbon

In one tracer experiment glucose is labeled with C13 at its C1 [the carbon one, (which carbon

In one tracer experiment glucose is labeled with C13 at its C1 [the carbon one, (which carbon
C1?)]. The glucose is used to feed yeast Saccharomyces cerevisiae that ferments it into
ethanol. Assuming all glucose fed becomes fermented to eth

minjai0322   993   1 Reply

bio_man

Multiple choice question for glycolysis

Multiple choice question for glycolysis

In glycolysis, each glucose molecule produces how many molecules of pyruvate?
(a) 0
(b) 1
(c) 2
(d) 3
(e) none of the above


The energy from glycolysis is preserved as (mark all that are correct)
(a) ATP

minjai0322   5134   1 Reply

bio_man

Listed below are the redox potential of “half reactions” of some reactions involved in electron ...

Listed below are the redox potential of “half reactions” of some reactions involved in electron ...

Listed below are the redox potential of “half reactions” of some reactions involved in electron transfer. Rank them according to the sequence of their position in transferring electrons from a high energetic state to a lower one.

Half reac

minjai0322   1527   1 Reply

bio_man

TCA cycle

TCA cycle

List two of the cofactors involved in the reaction catalyzed by pyruvate dehydrogenase. What is a cofactor of an enzyme?

minjai0322   792   1 Reply

bio_man

Oxidative Phosphorylation

Oxidative Phosphorylation

When dinitrophenol (DNP) is added to mitochondria, the inner membrane becomes permeable to protons.
(a) How will the electrochemical proton gradient change in response to DNP?
(b) DNP was one described as a weight loss drug. How would it pr

minjai0322   1197   1 Reply

bio_man

alpha helices and beta sheets

alpha helices and beta sheets

the amino acid with a distinct locked structure which interrupts alpha helices and beta sheets is called....................?

saab   3072   1 Reply

bio_man

Help Urgent!

Help Urgent!

The chemiosmotic theory involves the production of a(n) _________gradient that is then dissipated through a specific membrane protein.

ATP synthatase has _______active site(s) for synthesis of ATP

Complex IV provides sufficient

alatq33   913   1 Reply

bio_man

Metabolic Flux Analysis

Metabolic Flux Analysis

Some yeast cells growing on glucose can mix aerobic and anaerobic metabolism, that is, they consume oxygen and produce ethanol simultaneously when the O2 supply is restricted. The pathways for aerobic metabolism and ethanol production are both shown.

minjai0322   851   1 Reply

bio_man

Glycolysis

Glycolysis

Suppose 100 moles of glucose is broken down to pyruvate by glycolysis, and the pyruvate is then converted back to glucose by gluconeogenesis using the products from glycolysis. How many moles of glucose can be regenerated? First write down the stoich

minjai0322   838   1 Reply

minjai0322

Multiple choice quesions for Pentose Phosphate Pathway

Multiple choice quesions for Pentose Phosphate Pathway

 The process of making glucose when cells are grown on other sources (like amino acids) is called
(a) gluconeogenesis
(b) glucogenesis
(c) neoglucogenesis
(d) glucogenetics
(e) none of the above


 The stoichio

minjai0322   3617   1 Reply

bio_man

Glycolysis

Glycolysis

Suppose 100 moles of glucose is broken down to pyruvate by glycolysis, and the pyruvate is then converted back to glucose by gluconeogenesis using the products from glycolysis. How many moles of glucose can be regenerated? First write down the stoich

minjai0322   1162   1 Reply

bio_man