Upper-Year Courses

12 years ago

Use the following gene in protein synthesis:

Use the following gene in protein synthesis:
TAGGACCATTTTAGCCCC
o Show the mRNA.
o Show the tRNA.
o Name the amino acids that will be placed in the polypeptide chain

help

domenicog 2773 1 Reply

11 years ago

(surface plasmon resonance) which method are helpful for reduce negative curve?

1X tbs w/ sample
(1X TBS buffer : 90% DW+10% 10X TBS)

<1>
sample 100ul(diluted at DW until 1000X)
DW 800ul
10X TBS 100ul

it's my co-worker's opinion. he said "sample in DW, then when you

Koreanraichu 778 N/A

Koreanraichu

7 years ago

-20 microbial glycerol stocks

hello,

I am keeping microbial stocks in 25% glycerol at -20 (-80 not available) for a few months. but I am noticing that the stocks are remaining liquid. will this affect the viability of the cells ? if so what can I do without repeating t

tahah 1332 N/A

tahah

7 years ago

3T3 l1 cell differentiation culture

the fat accumulation is not enough after differentiation culture, can someone share the experience the culture experience?

Jamesmy 956 9 Replies

10 years ago

96 well plate cross contamination

I am using a 96 well plate to test antibiotic semsitivity if bacteria but after incubation, i found bacteria even in the control (i.e the one not containing bacteria / blank). I am suspecting that while incubation, the condensation that formed on the

micro 1141 3 Replies

10 years ago

A&P 2 Lab Effect of Exercise on Arterial Pressure and Vascular Resistance

Laboratory Report
Activity:
Effect of Exercise on Arterial Pressure and Vascular Resistance

Predictions
Exercise will cause MAP to
increase
Exercise will cause systemic vascular resistance to
decrease
Exe

phxguy 16734 4 Replies

Takota

9 years ago

About molecular cloning, does anyone know about extraction DNA from polyacrylamide gel?

Hey everybody. I'm a final year student and I have to do some experiments with SSR (simple sequence repeat) indicator. Now I'm preparing to cloning some genes and I have some troubles. It's the DNA extraction stage, but from 6% silver- staining polya

oark 684 2 Replies

juliyav

11 years ago

ACCT410 Questions

E2-3   (Fund and Nonfund Accounts Identification) Indicate the fund or nonfund accounts that should be used to account for each of the following:
1.   Tax revenues restricted for road maintenance. Special Revenue Funds
2.   Resources restricted f

amashlee88 15041 2 Replies

Janie73004

9 years ago

activity 6

Did the depth of the breathing pattern change during rebreathing? (Carefully examine the tracing for rate and depth changes; the changes can be subtle). Explain your observations.

liltwinkle20 6068 1 Reply

13 years ago

Activity 6

Activity 6 What happened to the pump’s rate as its stroke volume was
increased?
Using your simulation results as a basis for your answer, explain why an athlete’s resting heart rate might be lower than
that of the average person.

kmuhs 891 1 Reply

12 years ago

activity 6: comparitive spirometry

activity 6: comparitve spirometry:

23) what effect did acute asthma attack breathing have on FVC and FEV1?

25) describe the effect that the inhaler medication had on the FVC anf FEV1

26) did the values return to norma

birdistheword 1833 2 Replies

12 years ago

Aggregation of Bacteria

Why does ammonium sulfate aggregate bacteria in the SAT (salt aggregation test)?

1 2

micro 1652 17 Replies

micro

10 years ago

alcohol dehydrogenase assay, how do you know when things go wrong?

the actual question reads:
how would you know if the reaction preparation of a alcohol dehydrogenase has been compromised?

lerado1480t 461 1 Reply

smn

11 years ago

Algebra problem, finding out the total amount of Elisa's paycheck...?

Elisa got a paycheck from her part-time job. She spent some getting dinner, some on a movie, and some on clothes. She deposited the rest in her savings account. The total amount she spent was $148. The amount she saved was 20% more than the amount sh

irina 396 1 Reply

tonigra

11 years ago

Alternative method to pull down assay for protein-protein interactions?

I was wondering what other methods exist in addition to GSP-fusion/pull down assay and mass spec. to determine interaction of one protein with the other. I won't be able to perform crystallography, so that is not an option.

Is there anything else?

illicitf8 645 1 Reply

micro_2012

11 years ago

anatomy and physiology

stimulation of the beta receptors on the heart mucles cells results in ?

berfrye 1312 3 Replies

12 years ago

anatomy and physiology

Hi,can someone please answer this for me.thanks in advance

Muscarinic receptors...?
A) are activated by acetylcholine
B) are located on the surface of ganglion cells in both the sympathetic and parasympathetic n

berfrye 1508 4 Replies

berfrye

12 years ago

anatomy and physiology

does any one have answers to physioex 8.0 for A&P exercise 5,
need help please for activities 1-3

berfrye 979 1 Reply

MedicLife

12 years ago

Anatomy and Physiology Help Renal System NEED HELP FAST.

Can some one please help me with these questions?

What is the maximum osmolality of the interstitial fluid?

What is the renal threshold of glucose?

What factors result in dilute urine?

Thank you all so muc

llscott777 3529 5 Replies

12 years ago

anatomy and physiology multiple choice questions

someone kindly answer these two please

1. Blood pressure increases with all of the following, except?
a. increased cardiac output
b. peripheral resist

berfrye 3745 2 Replies

berfrye

12 years ago

Animal-Derived Laboratory Reagents

I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These inc

southtowns18 1074 1 Reply

lvnorbust

11 years ago

Any body familiar with the Elisa test? Pls help?

In the ELISA test one usually test by groups of three.Why did you assay your samples in triplicate? thanks ahead of time

Rizzlefritz 454 2 Replies

riyaghuman

11 years ago

apoptotic cell identification

Hello everyone,

What assays can we use to detect apoptosis in cells?
I know of the annexin 5 caspase assay. But what other methods are available?
Does trypan blue help identify dead cells? What about flow cytometry?

ehd123 778 3 Replies

9 years ago

Are M.D.'s scientists?

Don't get me wrong, being a doctor has got to be one of the hardest and most important professions there is. However, science in terms of professional research revolves around falsifying hypotheses through experiments which leads to innovation. Rathe

teom 787 5 Replies

JulieT

11 years ago

At what pH did you see the highest activity of salivary amylase? Why?

The following questions refer to Activity 1: Assessing Starch Digestion by Salivary Amylase.
1. At what pH did you see the highest activity of salivary amylase? Why?
2. How do you know that the amylase did not have any contaminating malto

AMIRA11 5296 4 Replies

12 years ago

Bacteria Absorbance

If you need to monitor bacterial growth by UV absorbance at 600 nm, do you take a small quantity from the culture and measure its absorbance every few hours and then discard it? Also, say after 24 hrs incubation you need to adjust the absorbance to a

micro 1620 1 Reply

micro

10 years ago

Bacterial Quantities

When a protocol instructs to transfer 10 mL of bacteria, what does it mean exactly? I imagine it is being assumed that bacteria is cultured in broth but then again, how is 10 mL a quantity for bacteria? Shouldn't the optical density be stated? I

micro 1125 9 Replies

mikael

10 years ago

Bees

Squar root of 87?

How to desect a bee hive

Skylerprue 542 1 Reply

9 years ago

Best host for transduction of the camR gene

You are given three different strains of S. enterica for a transduction experiment. Strain 1 has a wild-type phenotype, strain 2 has a defect in oligosaccharide synthesis and strain 3 is defective in Histidine synthesis? Which of these s

karmahone 718 2 Replies

7 years ago

Biological vs. Technical replicates and data processing, so confused!

How do you combine data from biological replicates that contain technical replicates into one set of data that represents an average for a repeated experiment with a n of > 3?

Example: Say I have cancer line X and drug Y. What I do:

CityD 996 1 Reply

colleen

11 years ago

biology lab 2 alters and alters

mitotic index
how to calculate the mitotic index?

judithlouis 1735 2 Replies

shawnep99

12 years ago

Biotechnology - chapter 20

whats the differences between bacteria express system and eukaryotic cloning and express system?

abertista 1924 1 Reply

habiba

8 years ago

Blood Pressure

What are the factors that trigger high blood pressure?

JC 1252 2 Replies

12 years ago

Caffeine and LB Agar

I'm doing an experiment that requires me to plate caffeine. At which point do I add the caffeine to the agar? Thanks for any help

michaelc1982 656 1 Reply

10 years ago

Caffeine experiment

I'm looking to set up an experiment to see the effects of caffeine on bacteria. I'm looking for some advice on experimental design and how to set it up.

michaelc1982 568 1 Reply

10 years ago

Calculating % Oxidation

In the biochemistry experiment, I measured the absorbance value of metal solutions.

I understand the concept that lower number of absorbance, the more oxidation
But I need to show the graph, absorbance vs % oxdiation

I h

kayleigh 515 3 Replies