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Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?
Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?
I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability to sequence from purified plasmid from 1 white cell.
Upper-Year Courses   !JRon@n   709   5 years ago
Suggest a laboratory test to distinguish between different elements.?
Suggest a laboratory test to distinguish between different elements.?
Hi, I need help with a Chemistry Lab question.  It asks to suggest a simple laboratory test to distinguish between acetone, carbon tetrachloride, cyclohexne, ethyl ether, and ethanol.
Introductory Courses   irina   372   rivera5454   6 years ago
What other oil can we use in place of immersion oil in light microscopy?
What other oil can we use in place of immersion oil in light microscopy?
And also can you please tell me what would happen if I use the oil with an ordinary dry objective lens instead of an immersion lens?
Introductory Courses   irina   1239   2   6 years ago
How to accurately do Protein/Enzyme Assay Calculations?
How to accurately do Protein/Enzyme Assay Calculations?
This is for my Biochemistry of Macromolecules Lab.....
For determining enzyme concentration (umole/min) for M1, P1, P2, and S2 using the fact that 9OD=1 umole, does these calculations seem reasonable.
The OD's are: M1=.888, P1=.352, P2=.473, and S2= .034
I divided the OD's by nine to yield the umole units and then multiplied by the dilution factor 50 (20:1000) and .020 ml (amount o
Upper-Year Courses   irina   5876   illicitf8   6 years ago
Algebra problem, finding out the total amount of Elisa's paycheck...?
Algebra problem, finding out the total amount of Elisa's paycheck...?
Elisa got a paycheck from her part-time job. She spent some getting dinner, some on a movie, and some on clothes. She deposited the rest in her savings account. The total amount she spent was $148. The amount she saved was 20% more than the amount she spent on dinner and a movie. The amount she spent on shopping was $16 more than the amount she saved. What was the total amount of he
Upper-Year Courses   irina   336   tonigra   6 years ago
How to calculate the total energy in a venturi flume?
How to calculate the total energy in a venturi flume?
I am doing a lab report for university and it involves a venturi flume and broad-crested weir (2 separate experiments) and need to calculate the total energy upstream of the obstruction and same downstream to calculate the energy loss.  However, I have more than one "total energy" reading for them both and was wondering how to find the total energy to do the question.  Do I average
Upper-Year Courses   irina   635   HTML   5 years ago
after receving a shot for varicella (chicken pox) how long do I have to wait to get a blood titre?
after receving a shot for varicella (chicken pox) how long do I have to wait to get a blood titre?
i need to have this doen for practice placement at college any help would be greatly appreciated. i had one done a titre, it was undetected..because im immune, but the college is makuing me get a shot and titre need info asap thank you
Feeling sick?   irina   426   Firedancer20   6 years ago
Is it possible for Bill Clinton to be drafted to run for president by popular demand?
Is it possible for Bill Clinton to be drafted to run for president by popular demand?
U.S needs Bill Clinton to be in the White House. How can we make it happen?
Off-Topic Room   irina   895   12   tommyo0729   6 years ago
What are useful things a tablet can do?
What are useful things a tablet can do?
I'm thinking of getting a tablet ( like the nexus 7 or iPad mini if it comes out). But what are some things I could do on one (other than games)?
Off-Topic Room   irina   567   2   fireguy1   6 years ago
What's the difference between the BlackBerry Curve 8310 and the BlackBerry Curve 8330?
What's the difference between the BlackBerry Curve 8310 and the BlackBerry Curve 8330?
I have AT&T and they don't have the BlackBerry Curve 8330 yet but they have the BlackBerry Curve 8310. What's the difference, they both look the same.
Off-Topic Room   irina   1010   5   duddy   6 years ago
AP chemistry studying please help?
AP chemistry studying please help?
Studying for the AP exam and can't figure out how to do these sorts of problems:

For a first order reaction with a rate constant k = 3.13 x 102 s-1, how long does it take for the concentration of the only reactant to become 40% of the original amount?
Academic Guidance   irina   620   fistpumporgy   7 years ago
How cool is Chemistry?
How cool is Chemistry?
I have reciently started my A-Levels and have chosen chemistry along with biology and french. I love all my subjects but chemistry is amazing!

I have reciently been thinking about going into a chemistry based career but dont know what the job prospects would be for me say if I were to study Chemistry with French?
or would I be better studying Pharmacy, as I have always seemed to wa
Academic Guidance   irina   552   2   Fisk71   7 years ago
what are some tipes for studying math?
what are some tipes for studying math?
hellow guys hope ya all doing good, i am kind of lost in math i want to get As in exams unfortunatly, i get B or C, i know most of people are good in math so if your one of the math geek please let me know how to become good in math and also what are some tips? i can do anything to learn math i just dont like my idiot professor i have no idea what the hek is he talking about but its
Academic Guidance   irina   519   2   lfh   7 years ago
If math and science are complex why should we expect God to be easy?
If math and science are complex why should we expect God to be easy?
I've seen a lot of questions put here on Y!A dealing with"If God is real why this?" or "I don't understand this so it must be untrue" but we study math and science in school for years. If their is a God wouldn't he be more complex then these things?

Wouldn't questioning his existence warrant more than a superficial glance?

What would happen if we treated other things as SOME (not
Academic Guidance   irina   954   9   smkt025   7 years ago
If you are good in math can you give me some advice?
If you are good in math can you give me some advice?
Like any tips or anything for basic and up?
Or is there something special you do before you study math?
Academic Guidance   irina   1440   10   lfh   7 years ago
Do Astronomers need a PhD in Physics to be well paid?
Do Astronomers need a PhD in Physics to be well paid?
I know we need a PhD in Astronomy! But in Physics do we need a PhD to be well paid or a Master Degree is Fine? And in Maths?
Academic Guidance   irina   697   smmoh4   7 years ago
So, Innocence of Muslims, 2, 800, 000 (and change) views, 1 day. That whole 'violent rampage'?
So, Innocence of Muslims, 2, 800, 000 (and change) views, 1 day. That whole 'violent rampage'?
thing didn't really work too well did it? "Oh crap, this building is on fire. Quick, somebody throw gasoline on it!"

Idiots.
synopsis, no, but if the imams that control them are trying to censor criticism of Islam, they've failed miserably. In fact, the imams couldn't have gotten more people to watch the video if they paid them to do it. Almost 3 million hits in ONE DAY.
Off-Topic Room   !JRon@n   561   5   nyno2010   6 years ago
How does air pollution change the natural balance in the ecosystem (carbon, water, and nitrogen cycles)???HELP
How does air pollution change the natural balance in the ecosystem (carbon, water, and nitrogen cycles)???HELP
How does air pollution change the natural balance in the ecosystem (carbon, water, and nitrogen cycles)???HELP
Introductory Courses   #8   752   _vibram_   6 years ago
is a pharmacy technician certification good for all states or just where you took the test?
is a pharmacy technician certification good for all states or just where you took the test?
next year i will be applying to pharmacy school and i want to be a certified tech this summer before i apply so it will be on my CV. i live in one state and plan on taking the PTCE here, but currently go to college in another. i plan to work as tech in the state of my college.will i have to take the exam there?
Academic Guidance   #8   594   2   Tiffany_m15   6 years ago
is there a mathematical model of hepatitis viral b?which is it?
is there a mathematical model of hepatitis viral b?which is it?
is there a mathematical model of hepatitis viral b?which is it?
Feeling sick?   $abood$   387   robinson12   7 years ago
I think I'm loosing hair on my left eyebrow, what's the possible causes?
I think I'm loosing hair on my left eyebrow, what's the possible causes?
I'm male, 21. There's a bald little patch on my left eyebrow. What's going on, the right one still bushy and nice. I don't pluck eyebrows, i'm a guy. I don't have bald genes in my family. My hair is quite a lot, except my left eyebrow, it seems that the little bald patch is growing. Please help, I'm kinda scare, I don't have a lot of money for doctors.
NO please don't give me some B
Feeling sick?   000127629   3966   2   123boy   6 years ago
what major would help me score high on the MCAT?
what major would help me score high on the MCAT?
santa clara university offers an actual pre-medicine degree.or should i take something in biological sciences. or would a biomedical degree be best instead?
Academic Guidance   003200222   567   3   11vizzinim   6 years ago
What do you take if your allergic to all statins and have a genetic problem with cholesterol?
What do you take if your allergic to all statins and have a genetic problem with cholesterol?
Im allergic to all the statins, and need something because I have the genetic family cholesterol.
Feeling sick?   007   765   1023ersin   6 years ago
What are signs of an allergic reaction to face wash?
What are signs of an allergic reaction to face wash?
I bought the Avalon Vitamin C face wash 2 days ago. First time I tried it, nothing weird happened(and I used a lot), second time I got redness on my cheeks and nose area(quite possibly from scrubbing to much), and third time I got the slightest bit of redness in the same place. It doesn't hurt or anything. Is this an allergic reaction? Will it really matter if I keep using it?
Feeling sick?   007   912   3   1 jersey girl   6 years ago
Is it possible to be allergic to milk but still able to eat chocolate?
Is it possible to be allergic to milk but still able to eat chocolate?
My childhood friend, Isabelle, said she is allergic to milk, but she can still eat chocolate. How is this possible?
Feeling sick?   007   459   7   1 jersey girl   6 years ago
Would calculus based physics better prepare you more for the MCAT than general physics?
Would calculus based physics better prepare you more for the MCAT than general physics?
I am aware that there isn't any calculus on the MCAT exam. But I have been told different things concerning the physics section of the exam. Some people say that the calculus based allows you to better understand the in-depth concepts of physics, to work the calculations etc. Others have said that the MCAT mostly follow the general physics curriculum, so gen. physics is enough.

Th
Academic Guidance   007   743   2   100442937   6 years ago
Is it possible to score above a 32 on the MCAT without doing one of those $2000 courses?
Is it possible to score above a 32 on the MCAT without doing one of those $2000 courses?
Is it possible to score above a 32 on the MCAT without doing one of those $2000 courses?
Academic Guidance   007   305   1234567   6 years ago
Is studying Latin good for preparation for the Verbal part of MCAT?
Is studying Latin good for preparation for the Verbal part of MCAT?
I wanted to know if individuals who study Latin have an edge on doing well on the Verbal portion of the MCAT or medical school in general.  Thanks in advance.
Academic Guidance   007   708   3   SmokeyV4L   6 years ago
How does tuberculosis attack the body and cause symtoms?
How does tuberculosis attack the body and cause symtoms?
hey guys im doing a science project on tuberculosis and:
How does the pathogen cause symtoms?
How does the pathogen spread?
What is its habitat?
What is its life cycle? ie. how does it reproduce, and the stages
Thank u so much!
Feeling sick?   007nahid   506   01svtcobra   6 years ago
I have so many medical issues, what have I done wrong?
I have so many medical issues, what have I done wrong?
First of all I am only 14 and I have alopecia areta which has wiped put all the hair in my body. Including my eyebrows and eyelashes, I can't cope with all the comments at school,
I have scleritis in one of my eyed causing me severe pain daily, so bad that I am literally living off pain killers every day, I have uveitis on my right eye again causing me pain. On top of the I have Idi
Feeling sick?   007nahid   481   002881   6 years ago
how much harder is the MCAT than the SAT?
how much harder is the MCAT than the SAT?
I know they're different, but do I need to work really harder for the MCAT than I did for the SAT, I didn't start preparing for the MCAT yet but I'm getting ready to start and just wanted to know how harder it is than the SAT.
Academic Guidance   007nahid   547   4   040408   6 years ago
Med school and mcat as international student?
Med school and mcat as international student?
Im in high school, going to do IB (living in Finland).
What is the system in US with med school.
How long does the study take and when is mcat taken?
Is it only provided in US?
Or can it be taken also in other countries like SAT?
Thank you.
Academic Guidance   007nahid   374   10684   6 years ago
Do I have the bald gene? I have telogen effluvium and mild alopecia areata?
Do I have the bald gene? I have telogen effluvium and mild alopecia areata?
My trichologist told me I have telogen effluvium and mild alopecia areata in the back and right side of my upper head.

I am 17 and a male. I have been losing LOADS of hair each day, and it's not balding in the male pattern baldness way at all.

I know to go bald maybe later on in life your hair needs to be sensitive to dht, does this mean my hair is sensitive to dht?

I read that t
Feeling sick?   00962265   478   2   170584   6 years ago
Pharmacology cases, 2 questions?
Pharmacology cases, 2 questions?
Q: A male patient has been taking erythromycin for the last 2 weeks for chest infection of mycoplasma pneumonia, what do you expect him to have as a side effect of the drug ?
a.Bleeding from his nose.
b.Aplastic Anemia.
c.alopecia(not sure) Jaundice.
d.None of the above.

Q: Which drugs should not be used for long term chemoprophylaxis ?
a.Ciprofloxacin.
b.Cephalosporins.
c.Aminogly
Feeling sick?   00destroyer1   441   000127629   6 years ago
Do medical schools even consider you if your MCAT score is way above a 30 but your gpa is a 3.4?
Do medical schools even consider you if your MCAT score is way above a 30 but your gpa is a 3.4?
Do medical schools even consider you if your MCAT score is way above a 30 but your gpa is a 3.4?? Which medical schools in the United States are the EASIEST to get into??
NO I'm not Black.
Academic Guidance   00destroyer1   350   2   0923849023u59   6 years ago
Can I start to study for the MCAT now, and what are some good books for MCAT prep?
Can I start to study for the MCAT now, and what are some good books for MCAT prep?
oh and by the way im going to college i have 4 years so you think its good idea if i will study now?
Academic Guidance   00destroyer1   758   6   1006471   6 years ago
If I was a Patient with Alopecia What would be some symptons?
If I was a Patient with Alopecia What would be some symptons?
Im doing a project so yeaaa.

Umm Yepp
Feeling sick?   00Rex   369   2   007nahid   6 years ago
Why dont nucleoside analog antiviral drugs harm our own cells?
Why dont nucleoside analog antiviral drugs harm our own cells?
Why dont nucleoside analog antiviral drugs harm our own cells?
Feeling sick?   00Rex   446   2   123kks   6 years ago
A benign cavity lined with keratinizing epithelium and filled with sebum and epithelial debris is a:
A benign cavity lined with keratinizing epithelium and filled with sebum and epithelial debris is a:
I need help with these 10 questions:

1. Baldness is:
a. hyperhidrosis
b. hypertrichosis
c. albinism
d. alopecia

2. Inflammatory disease of the sebaceous glands characterized by papules, pustules, and inflamed nodules, and plugs of sebum that block pores is:
a. seborrheic keratosis
b. acne
c. impetigo
d. seborrheic keratosis

3. A benign cavity lined with keratinizing epitheliu
Feeling sick?   00Rex   587   123kid   6 years ago
How low ratio A260/230 can effect ligation and how can I increase it?
How low ratio A260/230 can effect ligation and how can I increase it?

probably I should add that these DNA samples were gel extracted using column, so there is absolutely impossible that they are protein contaminated...
Introductory Courses   0103325   416   buk280forlife   6 years ago
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